Comparison of the pathogenicity of two US porcine reproductive and respiratory syndrome virus isolates with that of the Lelystad virus.

P. G. Halbur, P. S. Paul, M. L. Frey, J. Landgraf, K. Eernisse, X. J. Meng, M. A. Lum, J. J. Andrews, J. A. Rathje

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Abstract

The Lelystad virus or one of two US isolates (VR2385, VR2431) of porcine reproductive and respiratory syndrome virus were given intranasally to 25 4-week-old cesarian-derived colostrum-deprived pigs. Pigs from these groups were necropsied at 1, 2, 3, 5, 7, 10, 15, 21, or 28 days postinoculation. The Lelystad virus and VR2431 induced mild transient pyrexia, dyspnea, and tachypnea. VR2385 induced labored and rapid abdominal respiration, pyrexia, lethargy, anorexia, and patchy dermal cyanosis. All three isolates induced multifocal tan-mottled consolidation involving 6.8% (n = 9; SEM = 3.4) of the lung for Lelystad, 9.7% (n = 9, SEM = 2.7) of the lung for VR2431, and 54.2% (n = 9, SEM = 4.4) of the lung for VR2385 at 10 days postinoculation. Characteristic microscopic lung lesions consisted of type 2 pneumocyte hypertrophy and hyperplasia, necrotic debris and increased mixed inflammatory cells in alveolar spaces, and alveolar septal infiltration with mononuclear cells. Lymphadenopathy with follicular hypertrophy, hyperplasia, and necrosis was consistently seen. Similar follicular lesions were also seen in Peyer's patches and tonsils. Lymphohistiocytic myocarditis and encephalitis were reproduced with all three isolates. Clinical respiratory disease and gross and microscopic lung lesion scores were considerably and significantly more severe in the VR2385-inoculated pigs. All three viruses were readily isolated from sera, lungs, and tonsils throughout the 28 days of the study. The lymphoid and respiratory systems have the most remarkable lesions and appear to be the major site of replication of these viruses. This work demonstrated a marked difference in pathogenicity of porcine reproductive and respiratory syndrome isolates.

Original languageEnglish (US)
Pages (from-to)648-660
Number of pages13
JournalVeterinary pathology
Volume32
Issue number6
DOIs
StatePublished - Nov 1995

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Porcine respiratory and reproductive syndrome virus
Porcine reproductive and respiratory syndrome virus
Virulence
pathogenicity
lungs
lesions (animal)
Lung
Alveolar Epithelial Cells
Swine
tonsils
Palatine Tonsil
hypertrophy
hyperplasia
Hypertrophy
swine
fever
Hyperplasia
Fever
Porcine Reproductive and Respiratory Syndrome
pneumocytes

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Comparison of the pathogenicity of two US porcine reproductive and respiratory syndrome virus isolates with that of the Lelystad virus. / Halbur, P. G.; Paul, P. S.; Frey, M. L.; Landgraf, J.; Eernisse, K.; Meng, X. J.; Lum, M. A.; Andrews, J. J.; Rathje, J. A.

In: Veterinary pathology, Vol. 32, No. 6, 11.1995, p. 648-660.

Research output: Contribution to journalArticle

Halbur, PG, Paul, PS, Frey, ML, Landgraf, J, Eernisse, K, Meng, XJ, Lum, MA, Andrews, JJ & Rathje, JA 1995, 'Comparison of the pathogenicity of two US porcine reproductive and respiratory syndrome virus isolates with that of the Lelystad virus.', Veterinary pathology, vol. 32, no. 6, pp. 648-660. https://doi.org/10.1177/030098589503200606
Halbur, P. G. ; Paul, P. S. ; Frey, M. L. ; Landgraf, J. ; Eernisse, K. ; Meng, X. J. ; Lum, M. A. ; Andrews, J. J. ; Rathje, J. A. / Comparison of the pathogenicity of two US porcine reproductive and respiratory syndrome virus isolates with that of the Lelystad virus. In: Veterinary pathology. 1995 ; Vol. 32, No. 6. pp. 648-660.
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