Comparison of the intravenous and intraperitoneal routes of administration of tritiated thymidine in studies of cell production in the gastrointestinal tract of the rat

S. H. Fatemi, G. E. Cullan, G. M. Cullan, John G Sharp

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Incorporation of3H-Thymidine into DNA-synthesizing cells of the gastrointestinal tract of the rat was examined following administration of the isotope by intraperitoneal and intravenous routes. Estimates of whole tissue incorporation expressed as DPM/mg dry weight and of proliferating cells expressed as DPM/crypt or gland in the different segments of the gut indicated no differences in the degree of3H-TdR uptake into DNA following intraperitoneal or intravenous routes of administration. The possibility of misdirected I.P. injections was examined following injection of3H-TdR into the cecum or bladder. DPM/mg wet weight of gastrointestinal tissues indicated reduction in the uptake of3H-TdR into DNA of intestinal tissues following intracecal and intrabladder administration of3H-Tdr. The intraperitoneal route of administration of3H-TdR appears to be equally effective in the distribution of the isotope into different segments of the gut when compared to the intravenous route and is a more convenient method in studies of cell production in the gastrointestinal tract of the rat.

Original languageEnglish (US)
Pages (from-to)69-76
Number of pages8
JournalVirchows Archiv B Cell Pathology Including Molecular Pathology
Volume48
Issue number1
DOIs
Publication statusPublished - Jan 1 1985

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Keywords

  • Gastrointestinal tract
  • Routes of delivery
  • Tritiated thymidine

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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