Comparison of the antiproliferative effects of transforming growth factor-β, N,N-dimethylformamide and retinoic acid on a human colon carcinoma cell line

Naseema M. Hoosein, Diane E. Brattain, Mary K. McKnight, Karla E. Childress, Subhas Chakrabarty, Michael G. Brattain

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

In this study we have compared the anti-proliferative effects of transforming growth factor-β (TGF-β), N,N-dimethylformamide (DMF) and retinoic acid (RA) on a moderately-differentiated colon carcinoma cell line (JVC). TGF-β, DMF and RA inhibited the anchorage-independent growth and induced morphological changes in JVC cells. EC50 values of 40 pM TGF-β, 0.5% DMF and 5 nM RA were obtained for growth inhibition. In addition all three agents enhanced cellular fibronectin levels in a time- and dose-dependent manner. Inhibition of cell proliferation as well as fibronectin induction by all three agents were reversible. Combinations of any two agents at suboptimal doses, added simultaneously to JVC cells gave additive inhibitory response on growth. These data indicate that the effects of TGF-β in this colon carcinoma cell line are similar to those of the two differentiation promoting agents DMF and RA.

Original languageEnglish (US)
Pages (from-to)219-232
Number of pages14
JournalCancer Letters
Volume40
Issue number2
DOIs
Publication statusPublished - Jun 15 1988

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Keywords

  • Colon carcinoma cell line
  • Dimethylformamide
  • Fibronectin production
  • Retinoic acid
  • Transforming growth factor β

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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