Comparison of techniques for growing small bowel neomucosa

Jon S Thompson, Jon A. Vanderhoof, Dean Lowell Antonson, James R. Newland, Paul E. Hodgson

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Small bowel neomucosa has been grown on a variety of surfaces. The purpose of this study was to compare the rate of growth and function of neomucosa on colon serosa (CS) and abdominal wall muscle (AM) in New Zealand white rabbits. The terminal ileum was incised for 5 cm and patched with either adjacent CS (23 animals) or AM (19 animals) to create a 2 × 5-cm defect. Gross and histologic examinations of the specimens at 1, 2, 4, and 8 weeks revealed that the rate of growth was similar in both groups. There was minimal lateral ingrowth at 2 weeks, nearly complete coverage of the defects at 4 weeks, and complete coverage of the defect at 8 weeks in more than 85% of animals with mature villi and muscularis mucosae. The ileal diameter at the site of patching increased in both groups from 11.9 ± 2.6 to 16.3 ± 3.2 mm in the CS group and 11.3 ± 2.5 to 15.1 ± 1.8 mm in the AM group (P < 0.01). Glucose uptake was similar in both groups being 65.4 ± 24.1% of control in the CS group and 73.9 ± 29.8% in the AM group. Brush border enzyme activity of sucrase, maltase, and lactase was similar to controls in the AM group but in the CS group activity of sucrase and maltase were significantly less than controls (P < 0.01). Average body weight was increased postoperatively in both groups. There was one anastomotic leak in each group and two cases of partial intestinal obstruction in the abdominal wall group. Both colon serosa and abdominal wall musculature will support the growth of functioning neomucosa with a similar rate of growth and quality of the neomucosa. The use of either in clinical situations should be determined by availability and technical considerations.

Original languageEnglish (US)
Pages (from-to)401-406
Number of pages6
JournalJournal of Surgical Research
Volume36
Issue number4
DOIs
StatePublished - Jan 1 1984

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Serous Membrane
Abdominal Wall
Abdominal Muscles
Colon
Sucrase
alpha-Glucosidases
Growth
Lactase
Anastomotic Leak
Intestinal Obstruction
Microvilli
Ileum
Mucous Membrane
Body Weight
Rabbits
Glucose
Enzymes

ASJC Scopus subject areas

  • Surgery

Cite this

Thompson, J. S., Vanderhoof, J. A., Antonson, D. L., Newland, J. R., & Hodgson, P. E. (1984). Comparison of techniques for growing small bowel neomucosa. Journal of Surgical Research, 36(4), 401-406. https://doi.org/10.1016/0022-4804(84)90118-5

Comparison of techniques for growing small bowel neomucosa. / Thompson, Jon S; Vanderhoof, Jon A.; Antonson, Dean Lowell; Newland, James R.; Hodgson, Paul E.

In: Journal of Surgical Research, Vol. 36, No. 4, 01.01.1984, p. 401-406.

Research output: Contribution to journalArticle

Thompson, JS, Vanderhoof, JA, Antonson, DL, Newland, JR & Hodgson, PE 1984, 'Comparison of techniques for growing small bowel neomucosa', Journal of Surgical Research, vol. 36, no. 4, pp. 401-406. https://doi.org/10.1016/0022-4804(84)90118-5
Thompson, Jon S ; Vanderhoof, Jon A. ; Antonson, Dean Lowell ; Newland, James R. ; Hodgson, Paul E. / Comparison of techniques for growing small bowel neomucosa. In: Journal of Surgical Research. 1984 ; Vol. 36, No. 4. pp. 401-406.
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