Comparison of T-DNA oncogene complements of Agrobacterium tumefaciens tumor-inducing plasmids with limited and wide host ranges

W. G. Buchholz, M. F. Thomashow

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The T-DNA oncogene complements of the limited-host-range tumor-inducing plasmid pTiAg63 and the wide-host-range plasmid pTiA6 were compared. The resulting data indicate that pTiAg63 has DNA sequences related to most of the genes encoded by the oncogenic region, the T(L)-DNA, of pTiA6 and that these sequences are divided between two T-DNA regions, the T(A)-DNA, which encoded sequences related to pTiA6 genes 4 (the cytokinin independence gene) and 6a, as well as to a pTiA6 T(L)-DNA fragment had encoded gene 6b and a portion of gene 3, and the T(B)-DNA, which encoded sequences related to genes 1 and 2 (the auxin independence genes). Tumor tissues of Nicotiana rustica incited by Agrobacterium tumefaciens harboring either pTiA6 or TiAg63 grew axenically in vitro on phytohormone-free medium. The morphologies of the tissues, however, differed; whereas those incited with pTiA6 grew as loose, friable, unorganized callus, the tumors incited by pTiAg63 grew as clumps of rootlike structures. Thus, the T-DNA oncogene complements of these plasmids were not equivalent. The results are discussed in relation to the A. tumefaciens host range.

Original languageEnglish (US)
Pages (from-to)319-326
Number of pages8
JournalJournal of bacteriology
Volume160
Issue number1
StatePublished - Jan 1 1984

Fingerprint

Agrobacterium tumefaciens
Host Specificity
Oncogenes
Plasmids
Genes
Neoplasms
Cytokinins
Indoleacetic Acids
Plant Growth Regulators
Bony Callus
T-DNA
Tobacco

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Cite this

Comparison of T-DNA oncogene complements of Agrobacterium tumefaciens tumor-inducing plasmids with limited and wide host ranges. / Buchholz, W. G.; Thomashow, M. F.

In: Journal of bacteriology, Vol. 160, No. 1, 01.01.1984, p. 319-326.

Research output: Contribution to journalArticle

@article{f9ea6e375e824d74ab660237bcb1d85b,
title = "Comparison of T-DNA oncogene complements of Agrobacterium tumefaciens tumor-inducing plasmids with limited and wide host ranges",
abstract = "The T-DNA oncogene complements of the limited-host-range tumor-inducing plasmid pTiAg63 and the wide-host-range plasmid pTiA6 were compared. The resulting data indicate that pTiAg63 has DNA sequences related to most of the genes encoded by the oncogenic region, the T(L)-DNA, of pTiA6 and that these sequences are divided between two T-DNA regions, the T(A)-DNA, which encoded sequences related to pTiA6 genes 4 (the cytokinin independence gene) and 6a, as well as to a pTiA6 T(L)-DNA fragment had encoded gene 6b and a portion of gene 3, and the T(B)-DNA, which encoded sequences related to genes 1 and 2 (the auxin independence genes). Tumor tissues of Nicotiana rustica incited by Agrobacterium tumefaciens harboring either pTiA6 or TiAg63 grew axenically in vitro on phytohormone-free medium. The morphologies of the tissues, however, differed; whereas those incited with pTiA6 grew as loose, friable, unorganized callus, the tumors incited by pTiAg63 grew as clumps of rootlike structures. Thus, the T-DNA oncogene complements of these plasmids were not equivalent. The results are discussed in relation to the A. tumefaciens host range.",
author = "Buchholz, {W. G.} and Thomashow, {M. F.}",
year = "1984",
month = "1",
day = "1",
language = "English (US)",
volume = "160",
pages = "319--326",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Comparison of T-DNA oncogene complements of Agrobacterium tumefaciens tumor-inducing plasmids with limited and wide host ranges

AU - Buchholz, W. G.

AU - Thomashow, M. F.

PY - 1984/1/1

Y1 - 1984/1/1

N2 - The T-DNA oncogene complements of the limited-host-range tumor-inducing plasmid pTiAg63 and the wide-host-range plasmid pTiA6 were compared. The resulting data indicate that pTiAg63 has DNA sequences related to most of the genes encoded by the oncogenic region, the T(L)-DNA, of pTiA6 and that these sequences are divided between two T-DNA regions, the T(A)-DNA, which encoded sequences related to pTiA6 genes 4 (the cytokinin independence gene) and 6a, as well as to a pTiA6 T(L)-DNA fragment had encoded gene 6b and a portion of gene 3, and the T(B)-DNA, which encoded sequences related to genes 1 and 2 (the auxin independence genes). Tumor tissues of Nicotiana rustica incited by Agrobacterium tumefaciens harboring either pTiA6 or TiAg63 grew axenically in vitro on phytohormone-free medium. The morphologies of the tissues, however, differed; whereas those incited with pTiA6 grew as loose, friable, unorganized callus, the tumors incited by pTiAg63 grew as clumps of rootlike structures. Thus, the T-DNA oncogene complements of these plasmids were not equivalent. The results are discussed in relation to the A. tumefaciens host range.

AB - The T-DNA oncogene complements of the limited-host-range tumor-inducing plasmid pTiAg63 and the wide-host-range plasmid pTiA6 were compared. The resulting data indicate that pTiAg63 has DNA sequences related to most of the genes encoded by the oncogenic region, the T(L)-DNA, of pTiA6 and that these sequences are divided between two T-DNA regions, the T(A)-DNA, which encoded sequences related to pTiA6 genes 4 (the cytokinin independence gene) and 6a, as well as to a pTiA6 T(L)-DNA fragment had encoded gene 6b and a portion of gene 3, and the T(B)-DNA, which encoded sequences related to genes 1 and 2 (the auxin independence genes). Tumor tissues of Nicotiana rustica incited by Agrobacterium tumefaciens harboring either pTiA6 or TiAg63 grew axenically in vitro on phytohormone-free medium. The morphologies of the tissues, however, differed; whereas those incited with pTiA6 grew as loose, friable, unorganized callus, the tumors incited by pTiAg63 grew as clumps of rootlike structures. Thus, the T-DNA oncogene complements of these plasmids were not equivalent. The results are discussed in relation to the A. tumefaciens host range.

UR - http://www.scopus.com/inward/record.url?scp=0021182160&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021182160&partnerID=8YFLogxK

M3 - Article

C2 - 6090421

AN - SCOPUS:0021182160

VL - 160

SP - 319

EP - 326

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 1

ER -