Comparison of six commercial ELISA kits for their specificity and sensitivity in detecting different major peanut allergens

Shyamali Jayasena, Mieke Smits, Daniëlle Fiechter, Aard De Jong, Julie Nordlee, Joe Baumert, Steve L. Taylor, Raymond H. Pieters, Stef J. Koppelman

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

Six commercial peanut enzyme-linked immunosorbent assay kits were assessed for their ability to recover peanut from the standard reference material 2387 peanut butter and also for their specificity in detecting four major peanut allergens, Ara h 1, Ara h 2, Ara h 3, and Ara h 6. The percentage recovery of peanut from peanut butter differed across different kits as well as at different sample concentrations. The highest recovery was observed with the Romer and R-Biopharm kits, while four other kits were found to underestimate the protein content of the reference peanut butter samples. Five of the kits were most sensitive in detecting Ara h 3 followed by Ara h 1, while hardly recognizing Ara h 2 and Ara h 6. The other kit showed the highest sensitivity to Ara h 2 and Ara h 6, while Ara h 1 and Ara h 3 were poorly recognized. Although Ara h 2 and Ara h 6 are known to be heat stable and more potent allergens, antisera specific to any of these four peanut proteins/allergens may serve as good markers for the detection of peanut residues.

Original languageEnglish (US)
Pages (from-to)1849-1855
Number of pages7
JournalJournal of Agricultural and Food Chemistry
Volume63
Issue number6
DOIs
StatePublished - Feb 18 2015

Fingerprint

allergens
Allergens
peanuts
peanut butter
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
Sensitivity and Specificity
Recovery
Immunosorbents
Butter
Immune Sera
Assays
Proteins
peanut protein
Enzymes
reference standards
antiserum
protein content
Arachis
heat

Keywords

  • Ara h 1
  • Ara h 2
  • Ara h 3
  • Ara h 6
  • ELISA
  • allergens
  • detection
  • immunoassay
  • peanut

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Comparison of six commercial ELISA kits for their specificity and sensitivity in detecting different major peanut allergens. / Jayasena, Shyamali; Smits, Mieke; Fiechter, Daniëlle; De Jong, Aard; Nordlee, Julie; Baumert, Joe; Taylor, Steve L.; Pieters, Raymond H.; Koppelman, Stef J.

In: Journal of Agricultural and Food Chemistry, Vol. 63, No. 6, 18.02.2015, p. 1849-1855.

Research output: Contribution to journalArticle

Jayasena, Shyamali ; Smits, Mieke ; Fiechter, Daniëlle ; De Jong, Aard ; Nordlee, Julie ; Baumert, Joe ; Taylor, Steve L. ; Pieters, Raymond H. ; Koppelman, Stef J. / Comparison of six commercial ELISA kits for their specificity and sensitivity in detecting different major peanut allergens. In: Journal of Agricultural and Food Chemistry. 2015 ; Vol. 63, No. 6. pp. 1849-1855.
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