Comparison of porcine reproductive and respiratory syndrome virus growth in media supplemented with fetal bovine serum or a serum replacement

Susan K. Schommer, Susan L. Carpenter, Prem S. Paul

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Commercially available serum replacements are often used in cell culture as a cheaper and less variable substitute for fetal bovine serum (FBS). The growth of porcine reproductive and respiratory syndrome virus (PRRSV) isolates in CRL11171 cells maintained in a medium supplemented with FBS was compared with virus propagation in the same cell line maintained in the same medium with a serum replacement. The PRRSV replicated significantly better when the cell culture medium was supplemented with FBS. The results of this study have implications for the use of serum replacement-supplemented medium for PRRSV diagnosis by virus isolation.

Original languageEnglish (US)
Pages (from-to)276-279
Number of pages4
JournalJournal of Veterinary Diagnostic Investigation
Volume13
Issue number3
DOIs
StatePublished - May 2001

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Porcine respiratory and reproductive syndrome virus
Porcine reproductive and respiratory syndrome virus
fetal bovine serum
blood serum
culture media
cell culture
Growth
Serum
viruses
Cell Culture Techniques
cell lines
Viruses
Culture Media
cells
Cell Line

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Comparison of porcine reproductive and respiratory syndrome virus growth in media supplemented with fetal bovine serum or a serum replacement. / Schommer, Susan K.; Carpenter, Susan L.; Paul, Prem S.

In: Journal of Veterinary Diagnostic Investigation, Vol. 13, No. 3, 05.2001, p. 276-279.

Research output: Contribution to journalArticle

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