Comparison of perfusates in the study of choline uptake by isolated rat livers

D. J. Tuma, Michael Floyd Sorrell, A. J. Barak

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

In a previous study it was shown that hepatic choline uptake is primarily a function of oxygen dependent choline oxidase activity. Studies measuring choline uptake demonstrated that the isolated perfused liver maintained with erythrocyte containing perfusates offered the most efficient conditions for this measurement. It was shown in guinea pig livers, essentially lacking in choline oxidase, that the oxygen carrying capacity of the medium is not critical to choline uptake. These studies suggest that when oxygen requiring systems are studied using the isolated perfused liver technique, ideally the perfusate should contain red blood cells.

Original languageEnglish (US)
Title of host publicationRES.COMMUN.CHEM.PATH.PHARMACOL.
Pages345-348
Number of pages4
Volume6
Edition1
StatePublished - 1973

Fingerprint

choline oxidase
Choline
Liver
Oxygen
Erythrocytes
Conservation of Natural Resources
Guinea Pigs

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Tuma, D. J., Sorrell, M. F., & Barak, A. J. (1973). Comparison of perfusates in the study of choline uptake by isolated rat livers. In RES.COMMUN.CHEM.PATH.PHARMACOL. (1 ed., Vol. 6, pp. 345-348)

Comparison of perfusates in the study of choline uptake by isolated rat livers. / Tuma, D. J.; Sorrell, Michael Floyd; Barak, A. J.

RES.COMMUN.CHEM.PATH.PHARMACOL.. Vol. 6 1. ed. 1973. p. 345-348.

Research output: Chapter in Book/Report/Conference proceedingChapter

Tuma, DJ, Sorrell, MF & Barak, AJ 1973, Comparison of perfusates in the study of choline uptake by isolated rat livers. in RES.COMMUN.CHEM.PATH.PHARMACOL.. 1 edn, vol. 6, pp. 345-348.
Tuma DJ, Sorrell MF, Barak AJ. Comparison of perfusates in the study of choline uptake by isolated rat livers. In RES.COMMUN.CHEM.PATH.PHARMACOL.. 1 ed. Vol. 6. 1973. p. 345-348
Tuma, D. J. ; Sorrell, Michael Floyd ; Barak, A. J. / Comparison of perfusates in the study of choline uptake by isolated rat livers. RES.COMMUN.CHEM.PATH.PHARMACOL.. Vol. 6 1. ed. 1973. pp. 345-348
@inbook{c653ee9423e94f2fa9166894b74e6e9f,
title = "Comparison of perfusates in the study of choline uptake by isolated rat livers",
abstract = "In a previous study it was shown that hepatic choline uptake is primarily a function of oxygen dependent choline oxidase activity. Studies measuring choline uptake demonstrated that the isolated perfused liver maintained with erythrocyte containing perfusates offered the most efficient conditions for this measurement. It was shown in guinea pig livers, essentially lacking in choline oxidase, that the oxygen carrying capacity of the medium is not critical to choline uptake. These studies suggest that when oxygen requiring systems are studied using the isolated perfused liver technique, ideally the perfusate should contain red blood cells.",
author = "Tuma, {D. J.} and Sorrell, {Michael Floyd} and Barak, {A. J.}",
year = "1973",
language = "English (US)",
volume = "6",
pages = "345--348",
booktitle = "RES.COMMUN.CHEM.PATH.PHARMACOL.",
edition = "1",

}

TY - CHAP

T1 - Comparison of perfusates in the study of choline uptake by isolated rat livers

AU - Tuma, D. J.

AU - Sorrell, Michael Floyd

AU - Barak, A. J.

PY - 1973

Y1 - 1973

N2 - In a previous study it was shown that hepatic choline uptake is primarily a function of oxygen dependent choline oxidase activity. Studies measuring choline uptake demonstrated that the isolated perfused liver maintained with erythrocyte containing perfusates offered the most efficient conditions for this measurement. It was shown in guinea pig livers, essentially lacking in choline oxidase, that the oxygen carrying capacity of the medium is not critical to choline uptake. These studies suggest that when oxygen requiring systems are studied using the isolated perfused liver technique, ideally the perfusate should contain red blood cells.

AB - In a previous study it was shown that hepatic choline uptake is primarily a function of oxygen dependent choline oxidase activity. Studies measuring choline uptake demonstrated that the isolated perfused liver maintained with erythrocyte containing perfusates offered the most efficient conditions for this measurement. It was shown in guinea pig livers, essentially lacking in choline oxidase, that the oxygen carrying capacity of the medium is not critical to choline uptake. These studies suggest that when oxygen requiring systems are studied using the isolated perfused liver technique, ideally the perfusate should contain red blood cells.

UR - http://www.scopus.com/inward/record.url?scp=0015833350&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015833350&partnerID=8YFLogxK

M3 - Chapter

VL - 6

SP - 345

EP - 348

BT - RES.COMMUN.CHEM.PATH.PHARMACOL.

ER -