Comparison of ovine lentivirus detection by conventional and recombinant serological methods

Jim Keen, Jimmy Kwang, Sergio Rosati

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Recombinant (r) transmembrane protein (TM), major capsid protein P25, and matrix protein P16 of ovine lentivirus (OLV) were used as solid phase antigens in enzyme-linked immunosorbent assays (ELISAs) for the detection of specific antibodies against OLV in sheep sera. Sensitivity, specificity, and agreement of these three recombinant assays were compared with each other and with two currently available conventional OLV serological assays, the agar gel immunodiffusion (AGID) test and a whole-virus (WV) ELISA. Field sera from a total of 412 Midwestern United States sheep were tested and compared by the five OLV detection methods, including visibly healthy sheep selected for public sale (Group A, n = 171), samples from a breeding flock of Finnsheep and Finn-cross ewes (Group B, n = 184) and moribund sheep with clinical signs associated with OLV (Group C, n = 57). The rTM ELISA was the most sensitive OLV detection assay, both overall and within each group. Sera from 48.1% ( 198 412) of field samples were rTM ELISA positive. By contrast, positive rates for the rP25, rP16, and WV ELISAs and AGID test were 34.2%, 32.3%, 36.9%, and 26.9%, respectively. The rTM ELISA reactivity was 36.8% for Group A sera, 50.0% for Group B sera, and 75.4% for Group C sera. Among the 21 Group C sheep possessing OLV lung lesions at necropsy, 20 (95.2%) were rTM ELISA positive. The greatest test agreement occurred between the rP25 and the rP16 ELISAs. The data suggest that the recombinant TM immunoassay is the most accurate and sensitive of the five methods evaluated for the detection of serum anti-OLV antibodies in sheep, both at the subclinical infection and overt clinical disease stages.

Original languageEnglish (US)
Pages (from-to)295-309
Number of pages15
JournalVeterinary Immunology and Immunopathology
Volume47
Issue number3-4
DOIs
StatePublished - Aug 1995

Fingerprint

Lentivirus
Sheep
enzyme-linked immunosorbent assay
sheep
Enzyme-Linked Immunosorbent Assay
immunodiffusion tests
transmembrane proteins
Serum
methodology
recombinant proteins
assays
agar
gels
Finnsheep
Immunodiffusion
viruses
antibodies
Midwestern United States
Ovine lentivirus
Agar

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)

Cite this

Comparison of ovine lentivirus detection by conventional and recombinant serological methods. / Keen, Jim; Kwang, Jimmy; Rosati, Sergio.

In: Veterinary Immunology and Immunopathology, Vol. 47, No. 3-4, 08.1995, p. 295-309.

Research output: Contribution to journalArticle

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