Comparison of genomic and proteomic data in recurrent airway obstruction affected horses using ingenuity pathway analysis®

Julien Racine, Vinzenz Gerber, Marybeth Miskovic Feutz, C. Paige Riley, Jiri Adamec, June E. Swinburne, Laurent L. Couetil

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Background: Recurrent airway obstruction (RAO) is a severe chronic respiratory disease affecting horses worldwide, though mostly in the Northern hemisphere. Environmental as well as genetic factors strongly influence the course and prognosis of the disease. Research has been focused on characterization of immunologic factors contributing to inflammatory responses, on genetic linkage analysis, and, more recently, on proteomic analysis of airway secretions from affected horses. The goal of this study was to investigate the interactions between eight candidate genes previously identified in a genetic linkage study and proteins expressed in bronchoalveolar lavage fluid (BALF) collected from healthy and RAO-affected horses. The analysis was carried out with Ingenuity Pathway Analysis® bioinformatics software.Results: The gene with the greatest number of indirect interactions with the set of proteins identified is Interleukin 4 Receptor (IL-4R), whose protein has also been detected in BALF. Interleukin 21 receptor and chemokine (C-C motif) ligand 24 also showed a large number of interactions with the group of detected proteins. Protein products of other genes like that of SOCS5, revealed direct interactions with the IL-4R protein. The interacting proteins NOD2, RPS6KA5 and FOXP3 found in several pathways are reported regulators of the NFκB pathway.Conclusions: The pathways generated with IL-4R highlight possible important intracellular signaling cascades implicating, for instance, NFκB. Furthermore, the proposed interaction between SOCS5 and IL-4R could explain how different genes can lead to identical clinical RAO phenotypes, as observed in two Swiss Warmblood half sibling families because these proteins interact upstream of an important cascade where they may act as a functional unit.

Original languageEnglish (US)
Article number48
JournalBMC Veterinary Research
Volume7
DOIs
StatePublished - Aug 15 2011

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Airway Obstruction
Proteomics
proteomics
Horses
Interleukin-4 Receptors
genomics
horses
interleukin-4
receptors
Proteins
proteins
Genetic Linkage
Bronchoalveolar Lavage Fluid
linkage (genetics)
genes
Interleukin-21 Receptors
immunologic factors
Genes
protein products
CC Chemokines

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Comparison of genomic and proteomic data in recurrent airway obstruction affected horses using ingenuity pathway analysis®. / Racine, Julien; Gerber, Vinzenz; Miskovic Feutz, Marybeth; Riley, C. Paige; Adamec, Jiri; Swinburne, June E.; Couetil, Laurent L.

In: BMC Veterinary Research, Vol. 7, 48, 15.08.2011.

Research output: Contribution to journalArticle

Racine, Julien ; Gerber, Vinzenz ; Miskovic Feutz, Marybeth ; Riley, C. Paige ; Adamec, Jiri ; Swinburne, June E. ; Couetil, Laurent L. / Comparison of genomic and proteomic data in recurrent airway obstruction affected horses using ingenuity pathway analysis®. In: BMC Veterinary Research. 2011 ; Vol. 7.
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