Comparison of FilmArray and quantitative real-time reverse transcriptase PCR for detection of zaire ebolavirus from contrived and clinical specimens

Timothy R. Southern, Lori D. Racsa, César G. Albariño, Paul D Fey, Steven Heye Hinrichs, Caitlin N. Murphy, Vicki L. Herrera, Anthony R. Sambol, Charles E. Hill, Emily L. Ryan, Colleen S. Kraft, Shelley Campbell, Tara K. Sealy, Amy Schuh, James C. Ritchie, G. Marshall Lyon, Aneesh K. Mehta, Jay B. Varkey, Bruce S. Ribner, Kent P. BrantlyUte Ströher, Peter Charles Iwen, Eileen M. Burd

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Rapid, reliable, and easy-to-use diagnostic assays for detection of Zaire ebolavirus (ZEBOV) are urgently needed. The goal of this study was to examine the agreement among emergency use authorization (EUA) tests for the detection of ZEBOV nucleic acids, including the BioFire FilmArray BioThreat (BT) panel, the FilmArray BT-E panel, and the NP2 and VP40 quantitative real-time reverse transcriptase (qRT) PCR assays from the Centers for Disease Control and Prevention (CDC). Specimens used in this study included whole blood spiked with inactivated ZEBOV at known titers and whole-blood, plasma, and urine clinical specimens collected from persons diagnosed with Ebola virus disease (EVD). The agreement for FilmArray and qRT-PCR results using contrived whole-blood specimens was 100% (6/6 specimens) for each ZEBOV dilution from 4×107 to 4×102 50% tissue culture infective dose (TCID50)/ml, as well as the no-virus negative-control sample. The limit of detection for FilmArray and qRT-PCR assays with inactivated ZEBOV, based on duplicate positive results, was determined to be 4×102 TCID50/ml. Rates of agreement between FilmArray and qRT-PCR results for clinical specimens from patients with EVD were 85% (23/27 specimens) for whole-blood specimens, 90% (18/20 specimens) for whole-blood specimens tested by FilmArray testing and matched plasma specimens tested by qRT-PCR testing, and 85% (11/13 specimens) for urine specimens. Among 60 specimens, eight discordant results were noted, with ZEBOV nucleic acids being detected only by FilmArray testing in four specimens and only by qRT-PCR testing in the remaining four specimens. These findings demonstrate that the rapid and easy-to-use FilmArray panels are effective tests for evaluating patients with EVD.

Original languageEnglish (US)
Pages (from-to)2956-2960
Number of pages5
JournalJournal of clinical microbiology
Volume53
Issue number9
DOIs
StatePublished - Sep 1 2015

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Ebolavirus
Democratic Republic of the Congo
Reverse Transcriptase Polymerase Chain Reaction
Real-Time Polymerase Chain Reaction
Ebola Hemorrhagic Fever
Nucleic Acids
Urine
Centers for Disease Control and Prevention (U.S.)
Limit of Detection
Emergencies
Viruses

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Comparison of FilmArray and quantitative real-time reverse transcriptase PCR for detection of zaire ebolavirus from contrived and clinical specimens. / Southern, Timothy R.; Racsa, Lori D.; Albariño, César G.; Fey, Paul D; Hinrichs, Steven Heye; Murphy, Caitlin N.; Herrera, Vicki L.; Sambol, Anthony R.; Hill, Charles E.; Ryan, Emily L.; Kraft, Colleen S.; Campbell, Shelley; Sealy, Tara K.; Schuh, Amy; Ritchie, James C.; Lyon, G. Marshall; Mehta, Aneesh K.; Varkey, Jay B.; Ribner, Bruce S.; Brantly, Kent P.; Ströher, Ute; Iwen, Peter Charles; Burd, Eileen M.

In: Journal of clinical microbiology, Vol. 53, No. 9, 01.09.2015, p. 2956-2960.

Research output: Contribution to journalArticle

Southern, TR, Racsa, LD, Albariño, CG, Fey, PD, Hinrichs, SH, Murphy, CN, Herrera, VL, Sambol, AR, Hill, CE, Ryan, EL, Kraft, CS, Campbell, S, Sealy, TK, Schuh, A, Ritchie, JC, Lyon, GM, Mehta, AK, Varkey, JB, Ribner, BS, Brantly, KP, Ströher, U, Iwen, PC & Burd, EM 2015, 'Comparison of FilmArray and quantitative real-time reverse transcriptase PCR for detection of zaire ebolavirus from contrived and clinical specimens', Journal of clinical microbiology, vol. 53, no. 9, pp. 2956-2960. https://doi.org/10.1128/JCM.01317-15
Southern, Timothy R. ; Racsa, Lori D. ; Albariño, César G. ; Fey, Paul D ; Hinrichs, Steven Heye ; Murphy, Caitlin N. ; Herrera, Vicki L. ; Sambol, Anthony R. ; Hill, Charles E. ; Ryan, Emily L. ; Kraft, Colleen S. ; Campbell, Shelley ; Sealy, Tara K. ; Schuh, Amy ; Ritchie, James C. ; Lyon, G. Marshall ; Mehta, Aneesh K. ; Varkey, Jay B. ; Ribner, Bruce S. ; Brantly, Kent P. ; Ströher, Ute ; Iwen, Peter Charles ; Burd, Eileen M. / Comparison of FilmArray and quantitative real-time reverse transcriptase PCR for detection of zaire ebolavirus from contrived and clinical specimens. In: Journal of clinical microbiology. 2015 ; Vol. 53, No. 9. pp. 2956-2960.
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AU - Southern, Timothy R.

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AU - Fey, Paul D

AU - Hinrichs, Steven Heye

AU - Murphy, Caitlin N.

AU - Herrera, Vicki L.

AU - Sambol, Anthony R.

AU - Hill, Charles E.

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AU - Campbell, Shelley

AU - Sealy, Tara K.

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AU - Lyon, G. Marshall

AU - Mehta, Aneesh K.

AU - Varkey, Jay B.

AU - Ribner, Bruce S.

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N2 - Rapid, reliable, and easy-to-use diagnostic assays for detection of Zaire ebolavirus (ZEBOV) are urgently needed. The goal of this study was to examine the agreement among emergency use authorization (EUA) tests for the detection of ZEBOV nucleic acids, including the BioFire FilmArray BioThreat (BT) panel, the FilmArray BT-E panel, and the NP2 and VP40 quantitative real-time reverse transcriptase (qRT) PCR assays from the Centers for Disease Control and Prevention (CDC). Specimens used in this study included whole blood spiked with inactivated ZEBOV at known titers and whole-blood, plasma, and urine clinical specimens collected from persons diagnosed with Ebola virus disease (EVD). The agreement for FilmArray and qRT-PCR results using contrived whole-blood specimens was 100% (6/6 specimens) for each ZEBOV dilution from 4×107 to 4×102 50% tissue culture infective dose (TCID50)/ml, as well as the no-virus negative-control sample. The limit of detection for FilmArray and qRT-PCR assays with inactivated ZEBOV, based on duplicate positive results, was determined to be 4×102 TCID50/ml. Rates of agreement between FilmArray and qRT-PCR results for clinical specimens from patients with EVD were 85% (23/27 specimens) for whole-blood specimens, 90% (18/20 specimens) for whole-blood specimens tested by FilmArray testing and matched plasma specimens tested by qRT-PCR testing, and 85% (11/13 specimens) for urine specimens. Among 60 specimens, eight discordant results were noted, with ZEBOV nucleic acids being detected only by FilmArray testing in four specimens and only by qRT-PCR testing in the remaining four specimens. These findings demonstrate that the rapid and easy-to-use FilmArray panels are effective tests for evaluating patients with EVD.

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