Comparison of Disposition Values Obtained by Two Assay Methods for Quinidine Gluconate in Patients with Ventricular Tachycardia

Bruce H. Ackerman, Keith M. Olsen, Eleanor E. Kennedy, E. Howard Taylor, Bai H. Chen, Drexel Jordan, Deborah J. Ackerman

Research output: Contribution to journalArticle

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Abstract

Eight patients with previously untreated ventricular tachycardia, age 48.54 ± 28.02 years (mean ± SD), were enrolled in a protocol evaluating the disposition of quinidine gluconate as determined by two assay methods. Patients received two infusions of 5 mg/kg over 30 minutes separated by 20–30 (24.9 ± 4.0) minutes of electrophysiologic testing. Blood samples were obtained at 0.17 hours and just prior to the second infusion, and then at 0.17, 0.25, 0.33, 1.0, 6.0, 12.0, and 24.0 hours after the second infusion. Paired serum samples were assayed for quinidine concentrations by fluorescence polarization immunoassay and high‐performance liquid chromatography. The two assays compared well, with a linear regression equation of Y = 0.927X + 0.247 with a correlation coefficient of 0.985. With the exception of the ß elimination rate constant and ß distribution volume, t test comparison of disposition values demonstrated no significant difference. Differences in the estimates of the ß elimination rate constant reflected differences in the two methods and indicated that even though both assays were comparable, subtle differences in specificity could be reflected in significant differences in this variable. 1989 Pharmacotherapy Publications Inc.

Original languageEnglish (US)
Pages (from-to)220-225
Number of pages6
JournalPharmacotherapy: The Journal of Human Pharmacology and Drug Therapy
Volume9
Issue number4
DOIs
StatePublished - Jan 1 1989

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Ventricular Tachycardia
Fluorescence Polarization Immunoassay
Quinidine
Liquid Chromatography
Publications
Linear Models
Drug Therapy
Serum
quinidine gluconate

ASJC Scopus subject areas

  • Pharmacology (medical)

Cite this

Comparison of Disposition Values Obtained by Two Assay Methods for Quinidine Gluconate in Patients with Ventricular Tachycardia. / Ackerman, Bruce H.; Olsen, Keith M.; Kennedy, Eleanor E.; Taylor, E. Howard; Chen, Bai H.; Jordan, Drexel; Ackerman, Deborah J.

In: Pharmacotherapy: The Journal of Human Pharmacology and Drug Therapy, Vol. 9, No. 4, 01.01.1989, p. 220-225.

Research output: Contribution to journalArticle

Ackerman, Bruce H. ; Olsen, Keith M. ; Kennedy, Eleanor E. ; Taylor, E. Howard ; Chen, Bai H. ; Jordan, Drexel ; Ackerman, Deborah J. / Comparison of Disposition Values Obtained by Two Assay Methods for Quinidine Gluconate in Patients with Ventricular Tachycardia. In: Pharmacotherapy: The Journal of Human Pharmacology and Drug Therapy. 1989 ; Vol. 9, No. 4. pp. 220-225.
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