Vergleich der Aktivität Nucleolus organisierender Regionen an Metaphasechromosomen embryonaler Gewebe und Fibroblastenkulturen der Wachtel (Coturnix coturnix japonica)

Translated title of the contribution: Comparison of activity of chromosomal nucleolus organizer regions in embryonic tissues and fibroblast cultures of Japanese quall

Kay-Uwe Wagner, Lutz Schüler, W. Saar, M. Apitz

Research output: Contribution to journalArticle

Abstract

The silver labelling technique by HOWELL and BLACK (1980) was used to stain active chromosomal nucleolus organizer regions (NOR) of embryonic tissues and fibroblast cultures in Japanese quail. Active rRNA gene clusters were localised always at very small microchromosomes, which were not distinguishable in their common morphology. A minimum of 2 and a maximum of 8 NORs were observed, however, over 94% of all spreads had 4 up to 6 active nucleolus organizer regions. Tissues of 2-d-old embryos embody with 5.12 (± 0.27) slightly more NORs that 4-d-old embryonic tissues (5.01 ± .26). No significant differences between these embryonic stages were calculated within all populations of Japanese quail (P > 0.05). Also, no difference in the mean number of active NORs between embryonic stages (5.07 ± 0.26) and fibroblast cultures of adult individuals (5.09 ± 0.20) was determined (P > 0.05). A significant decrease in the mitotic index from 2- to 4-d-old embryos was of no effect on the number and variation of active chromosomal nucleolus organizer regions. Earlier experiments at the same material showed a similar decrease of mitotic rate and expression of rRNA genes investigated in different parameters of nucleoli in interphase cells (WAGNER et al., 1994). Therefore, the present results indicate that, the regulation of gene expression is rather caused by each single rRNA Loci of all NORs than by "turn on" or "turn off" of an entire nucleolus organizer region.

Original languageGerman
Pages (from-to)58-64
Number of pages7
JournalArchiv fur Geflugelkunde
Volume60
Issue number2
StatePublished - Apr 1 1996

Fingerprint

Nucleolus Organizer Region
nucleolus organizer region
fibroblasts
Fibroblasts
Coturnix
ribosomal RNA
rRNA Genes
Embryonic Structures
labeling techniques
Mitotic Index
Interphase
gene expression regulation
Gene Expression Regulation
cell nucleolus
Multigene Family
Silver
multigene family
interphase
silver
Coloring Agents

ASJC Scopus subject areas

  • Food Animals
  • Animal Science and Zoology

Cite this

Vergleich der Aktivität Nucleolus organisierender Regionen an Metaphasechromosomen embryonaler Gewebe und Fibroblastenkulturen der Wachtel (Coturnix coturnix japonica). / Wagner, Kay-Uwe; Schüler, Lutz; Saar, W.; Apitz, M.

In: Archiv fur Geflugelkunde, Vol. 60, No. 2, 01.04.1996, p. 58-64.

Research output: Contribution to journalArticle

@article{d42e6fa9b07b4697a7b5f48d106767de,
title = "Vergleich der Aktivit{\"a}t Nucleolus organisierender Regionen an Metaphasechromosomen embryonaler Gewebe und Fibroblastenkulturen der Wachtel (Coturnix coturnix japonica)",
abstract = "The silver labelling technique by HOWELL and BLACK (1980) was used to stain active chromosomal nucleolus organizer regions (NOR) of embryonic tissues and fibroblast cultures in Japanese quail. Active rRNA gene clusters were localised always at very small microchromosomes, which were not distinguishable in their common morphology. A minimum of 2 and a maximum of 8 NORs were observed, however, over 94{\%} of all spreads had 4 up to 6 active nucleolus organizer regions. Tissues of 2-d-old embryos embody with 5.12 (± 0.27) slightly more NORs that 4-d-old embryonic tissues (5.01 ± .26). No significant differences between these embryonic stages were calculated within all populations of Japanese quail (P > 0.05). Also, no difference in the mean number of active NORs between embryonic stages (5.07 ± 0.26) and fibroblast cultures of adult individuals (5.09 ± 0.20) was determined (P > 0.05). A significant decrease in the mitotic index from 2- to 4-d-old embryos was of no effect on the number and variation of active chromosomal nucleolus organizer regions. Earlier experiments at the same material showed a similar decrease of mitotic rate and expression of rRNA genes investigated in different parameters of nucleoli in interphase cells (WAGNER et al., 1994). Therefore, the present results indicate that, the regulation of gene expression is rather caused by each single rRNA Loci of all NORs than by {"}turn on{"} or {"}turn off{"} of an entire nucleolus organizer region.",
author = "Kay-Uwe Wagner and Lutz Sch{\"u}ler and W. Saar and M. Apitz",
year = "1996",
month = "4",
day = "1",
language = "German",
volume = "60",
pages = "58--64",
journal = "Archiv fur Geflugelkunde",
issn = "0003-9098",
publisher = "Verlag Eugen Ulmer",
number = "2",

}

TY - JOUR

T1 - Vergleich der Aktivität Nucleolus organisierender Regionen an Metaphasechromosomen embryonaler Gewebe und Fibroblastenkulturen der Wachtel (Coturnix coturnix japonica)

AU - Wagner, Kay-Uwe

AU - Schüler, Lutz

AU - Saar, W.

AU - Apitz, M.

PY - 1996/4/1

Y1 - 1996/4/1

N2 - The silver labelling technique by HOWELL and BLACK (1980) was used to stain active chromosomal nucleolus organizer regions (NOR) of embryonic tissues and fibroblast cultures in Japanese quail. Active rRNA gene clusters were localised always at very small microchromosomes, which were not distinguishable in their common morphology. A minimum of 2 and a maximum of 8 NORs were observed, however, over 94% of all spreads had 4 up to 6 active nucleolus organizer regions. Tissues of 2-d-old embryos embody with 5.12 (± 0.27) slightly more NORs that 4-d-old embryonic tissues (5.01 ± .26). No significant differences between these embryonic stages were calculated within all populations of Japanese quail (P > 0.05). Also, no difference in the mean number of active NORs between embryonic stages (5.07 ± 0.26) and fibroblast cultures of adult individuals (5.09 ± 0.20) was determined (P > 0.05). A significant decrease in the mitotic index from 2- to 4-d-old embryos was of no effect on the number and variation of active chromosomal nucleolus organizer regions. Earlier experiments at the same material showed a similar decrease of mitotic rate and expression of rRNA genes investigated in different parameters of nucleoli in interphase cells (WAGNER et al., 1994). Therefore, the present results indicate that, the regulation of gene expression is rather caused by each single rRNA Loci of all NORs than by "turn on" or "turn off" of an entire nucleolus organizer region.

AB - The silver labelling technique by HOWELL and BLACK (1980) was used to stain active chromosomal nucleolus organizer regions (NOR) of embryonic tissues and fibroblast cultures in Japanese quail. Active rRNA gene clusters were localised always at very small microchromosomes, which were not distinguishable in their common morphology. A minimum of 2 and a maximum of 8 NORs were observed, however, over 94% of all spreads had 4 up to 6 active nucleolus organizer regions. Tissues of 2-d-old embryos embody with 5.12 (± 0.27) slightly more NORs that 4-d-old embryonic tissues (5.01 ± .26). No significant differences between these embryonic stages were calculated within all populations of Japanese quail (P > 0.05). Also, no difference in the mean number of active NORs between embryonic stages (5.07 ± 0.26) and fibroblast cultures of adult individuals (5.09 ± 0.20) was determined (P > 0.05). A significant decrease in the mitotic index from 2- to 4-d-old embryos was of no effect on the number and variation of active chromosomal nucleolus organizer regions. Earlier experiments at the same material showed a similar decrease of mitotic rate and expression of rRNA genes investigated in different parameters of nucleoli in interphase cells (WAGNER et al., 1994). Therefore, the present results indicate that, the regulation of gene expression is rather caused by each single rRNA Loci of all NORs than by "turn on" or "turn off" of an entire nucleolus organizer region.

UR - http://www.scopus.com/inward/record.url?scp=0347938885&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0347938885&partnerID=8YFLogxK

M3 - Article

VL - 60

SP - 58

EP - 64

JO - Archiv fur Geflugelkunde

JF - Archiv fur Geflugelkunde

SN - 0003-9098

IS - 2

ER -