Comparison of a direct radioimmunoassay with virus isolation in cell culture for detection of pseudorabies virus in tissues of infected swine

J. D. Neill, Clayton L Kelling, M. B. Rhodes

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A direct radioimmunoassay (DRIA) was developed for detection of pseudorabies virus (PRV) in tissue of pigs with clinical disease. Homogenates of tissues prepared from 289 swine were tested for PRV with the DRIA and virus-isolation technique (VIT). The virus was detected in 53 (18%) of the homogenates by both DRIA and VIT. Fifteen (5%) others were positive for PTV by VIT, but negative by DRIA. There were no samples negative by VIT and positive by DRIA. A total of 211 (77%) pigs with clinical PRV infection were negative in both DRIA and VIT. Although the VIT is more sensitive than DRIA, the DRIA possesses certain advantages which include reduced requirements for aseptic procedures and cell cultures, rapidity, and increased objectivity. Moreover, high levels of confidence could be attributed to DRIA-positive results, since DRIA-positive/VIT-negative results were not obtained.

Original languageEnglish (US)
Pages (from-to)708-710
Number of pages3
JournalAmerican journal of veterinary research
Volume43
Issue number4
StatePublished - Dec 1 1982

Fingerprint

Suid Herpesvirus 1
Suid herpesvirus 1
radioimmunoassays
Radioimmunoassay
cell culture
Swine
Cell Culture Techniques
isolation techniques
Viruses
viruses
swine
tissues
Virus Diseases

ASJC Scopus subject areas

  • veterinary(all)

Cite this

@article{336f063edd334b7f86a1a15f8a8c6cda,
title = "Comparison of a direct radioimmunoassay with virus isolation in cell culture for detection of pseudorabies virus in tissues of infected swine",
abstract = "A direct radioimmunoassay (DRIA) was developed for detection of pseudorabies virus (PRV) in tissue of pigs with clinical disease. Homogenates of tissues prepared from 289 swine were tested for PRV with the DRIA and virus-isolation technique (VIT). The virus was detected in 53 (18{\%}) of the homogenates by both DRIA and VIT. Fifteen (5{\%}) others were positive for PTV by VIT, but negative by DRIA. There were no samples negative by VIT and positive by DRIA. A total of 211 (77{\%}) pigs with clinical PRV infection were negative in both DRIA and VIT. Although the VIT is more sensitive than DRIA, the DRIA possesses certain advantages which include reduced requirements for aseptic procedures and cell cultures, rapidity, and increased objectivity. Moreover, high levels of confidence could be attributed to DRIA-positive results, since DRIA-positive/VIT-negative results were not obtained.",
author = "Neill, {J. D.} and Kelling, {Clayton L} and Rhodes, {M. B.}",
year = "1982",
month = "12",
day = "1",
language = "English (US)",
volume = "43",
pages = "708--710",
journal = "American Journal of Veterinary Research",
issn = "0002-9645",
publisher = "American Veterinary Medical Association",
number = "4",

}

TY - JOUR

T1 - Comparison of a direct radioimmunoassay with virus isolation in cell culture for detection of pseudorabies virus in tissues of infected swine

AU - Neill, J. D.

AU - Kelling, Clayton L

AU - Rhodes, M. B.

PY - 1982/12/1

Y1 - 1982/12/1

N2 - A direct radioimmunoassay (DRIA) was developed for detection of pseudorabies virus (PRV) in tissue of pigs with clinical disease. Homogenates of tissues prepared from 289 swine were tested for PRV with the DRIA and virus-isolation technique (VIT). The virus was detected in 53 (18%) of the homogenates by both DRIA and VIT. Fifteen (5%) others were positive for PTV by VIT, but negative by DRIA. There were no samples negative by VIT and positive by DRIA. A total of 211 (77%) pigs with clinical PRV infection were negative in both DRIA and VIT. Although the VIT is more sensitive than DRIA, the DRIA possesses certain advantages which include reduced requirements for aseptic procedures and cell cultures, rapidity, and increased objectivity. Moreover, high levels of confidence could be attributed to DRIA-positive results, since DRIA-positive/VIT-negative results were not obtained.

AB - A direct radioimmunoassay (DRIA) was developed for detection of pseudorabies virus (PRV) in tissue of pigs with clinical disease. Homogenates of tissues prepared from 289 swine were tested for PRV with the DRIA and virus-isolation technique (VIT). The virus was detected in 53 (18%) of the homogenates by both DRIA and VIT. Fifteen (5%) others were positive for PTV by VIT, but negative by DRIA. There were no samples negative by VIT and positive by DRIA. A total of 211 (77%) pigs with clinical PRV infection were negative in both DRIA and VIT. Although the VIT is more sensitive than DRIA, the DRIA possesses certain advantages which include reduced requirements for aseptic procedures and cell cultures, rapidity, and increased objectivity. Moreover, high levels of confidence could be attributed to DRIA-positive results, since DRIA-positive/VIT-negative results were not obtained.

UR - http://www.scopus.com/inward/record.url?scp=0020328881&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020328881&partnerID=8YFLogxK

M3 - Article

C2 - 6280535

AN - SCOPUS:0020328881

VL - 43

SP - 708

EP - 710

JO - American Journal of Veterinary Research

JF - American Journal of Veterinary Research

SN - 0002-9645

IS - 4

ER -