Comparative virulence of isolates of bovine viral diarrhea virus type II in experimentally inoculated six- to nine-month-old calves

Clayton L Kelling, David J Steffen, Christina L. Topliff, Kent M Eskridge, Ruben O. Donis, Deborah S. Higuchi

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Abstract

Objective - To determine the comparative virulence of 5 isolates of bovine viral diarrhea virus (BVDV) type II by inoculating 6- to 9-month-old beef calves with isolates originating from the tissues of cattle affected with naturally occurring, transient, acute, nonfatal infections or naturally occurring, peracute, fatal infections. Animals - 22 calves that were 6 to 9 months old. Procedure - The study used BVDV isolates 17011, 713, and 5521 that originated from fetuses aborted from cows with transient, nonfatal, acute BVDV infections and isolates 23025 and 17583 that originated from the tissues of cattle with peracute, fatal BVDV infections. Calves were allotted to 6 groups (1, mock-infected control calves [n = 2]; 2, inoculated with BVDV 17011 [4]; 3, inoculated with BVDV 713 [4]; 4, inoculated with BVDV 5521 [4]; 5, inoculated with BVDV 23025 [4]; and 6, inoculated with BVDV 17583 [4]). Rectal temperatures and clinical signs of disease were recorded daily. Total and differential WBC and platelet counts were performed. Histologic examination and immunohistochemical analysis were conducted to detect lesions and distribution of viral antigens, respectively. Results - Calves inoculated with BVDV 23025 or 17583 developed more severe clinical signs of disease (fever and diarrhea), more severe lymphopenia, and more severe lesions (alimentary epithelial necrosis, lymphoid depletion, and BVDV antigen deposition in lymphatic tissues), compared with calves inoculated with BVDV 713, 5521, or 17011. Conclusions and Clinical Relevance - Relative severity of experimentally induced infections corresponded to severity of clinical signs of naturally occurring infections with respective BVDV isolates.

Original languageEnglish (US)
Pages (from-to)1379-1384
Number of pages6
JournalAmerican journal of veterinary research
Volume63
Issue number10
DOIs
StatePublished - Oct 1 2002

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Bovine Viral Diarrhea Viruses
Bovine viral diarrhea virus
Virulence
virulence
calves
infection
Virus Diseases
Infection
lesions (animal)
Aborted Fetus
Lymphopenia
Viral Antigens
blood platelet count
viral antigens
cattle
Lymphoid Tissue
Platelet Count
leukocyte count
fever
fetus

ASJC Scopus subject areas

  • veterinary(all)

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Comparative virulence of isolates of bovine viral diarrhea virus type II in experimentally inoculated six- to nine-month-old calves. / Kelling, Clayton L; Steffen, David J; Topliff, Christina L.; Eskridge, Kent M; Donis, Ruben O.; Higuchi, Deborah S.

In: American journal of veterinary research, Vol. 63, No. 10, 01.10.2002, p. 1379-1384.

Research output: Contribution to journalArticle

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