Comparative studies on expression of tumor-associated antigens in human and induced pancreatic cancer in syrian hamsters

Hiroshi Egami, Yoshiyuki Takiyama, William G Chaney, Martin Cano, Hideki Fujii, Tsutoma Tomioka, Richard Metzgar, Parviz M. Pour

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The expression of blood-group-related antigens (BGRAs) in experimental primary pancreatic cancer induced by N-nitrosobis(2-oxopropyl)amine (BOP) treatment of Syrian hamsters and homologous subcutaneous transplants of this primary cancer in the cell line, PC-1, established from the primary cancer and intrapancreatic transplanted PC-1 cells were studied by histochemical and biochemical methods. Human primary pancreatic cancer; the human pancreatic cancer cell line, HPAF; and its subclones, CDU and CD18, also were studied on a comparative basis. Histochemical analysis of BGRAs demonstrated that A, B, H, Le b , Le x Le y , and T antigen were expressed both in vivo and in vitro in hamster and human materials in similar patterns. However, Le a , CA 19-9 and sialylated Tn antigens were not found in hamster-derived tissues. SDS-PAGE and Western blotting procedures using anti-A antigen revealed similar major bands in the membrane fractions of both human and hamster pancreatic cells between 97 and 200 kdalton. Among other human pancreatic cancerassociated antigens, TAG-72, CA 125, and 17-1A were detected immunohistochemically in the hamster tumors both in vivo and in vitro, in a pattern similar to that seen in human pancreatic cancer. Tumor antigen DU-PAN-2, associated with human pancreatic cancer, was found infrequently in hamster pancreatic cancer specimens. These results indicate that the experimental hamster pancreatic cancer model provides a unique tool for investigating antigenicity of pancreatic cancer, particularly in relation to diagnosis and therapy.

Original languageEnglish (US)
Pages (from-to)91-100
Number of pages10
JournalInternational Journal of Pancreatology
Volume7
Issue number1-3
DOIs
StatePublished - Aug 1 1990

Fingerprint

Mesocricetus
Neoplasm Antigens
Pancreatic Neoplasms
Cricetinae
nitrosobis(2-oxopropyl)amine
Blood Group Antigens
Lewis Blood-Group System
Antigens
Cell Line
Neoplasms
Viral Tumor Antigens
Allografts
Polyacrylamide Gel Electrophoresis
Western Blotting
Membranes

Keywords

  • Tumor immunology
  • blood group antigens
  • hamster
  • human
  • pancreatic cancer

ASJC Scopus subject areas

  • Oncology
  • Endocrinology
  • Gastroenterology

Cite this

Comparative studies on expression of tumor-associated antigens in human and induced pancreatic cancer in syrian hamsters. / Egami, Hiroshi; Takiyama, Yoshiyuki; Chaney, William G; Cano, Martin; Fujii, Hideki; Tomioka, Tsutoma; Metzgar, Richard; Pour, Parviz M.

In: International Journal of Pancreatology, Vol. 7, No. 1-3, 01.08.1990, p. 91-100.

Research output: Contribution to journalArticle

Egami, H, Takiyama, Y, Chaney, WG, Cano, M, Fujii, H, Tomioka, T, Metzgar, R & Pour, PM 1990, 'Comparative studies on expression of tumor-associated antigens in human and induced pancreatic cancer in syrian hamsters', International Journal of Pancreatology, vol. 7, no. 1-3, pp. 91-100. https://doi.org/10.1007/BF02924224
Egami, Hiroshi ; Takiyama, Yoshiyuki ; Chaney, William G ; Cano, Martin ; Fujii, Hideki ; Tomioka, Tsutoma ; Metzgar, Richard ; Pour, Parviz M. / Comparative studies on expression of tumor-associated antigens in human and induced pancreatic cancer in syrian hamsters. In: International Journal of Pancreatology. 1990 ; Vol. 7, No. 1-3. pp. 91-100.
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