Comparative studies of bacteria with an atomic force microscopy operating in different modes

A. V. Bolshakova, O. I. Kiselyova, A. S. Filonov, O. Yu Frolova, Y. L. Lyubchenko, I. V. Yaminsky

Research output: Contribution to journalArticle

130 Citations (Scopus)

Abstract

Escherichia coli bacterial cells of two strains JM109 and K12 J62 were imaged with atomic force microscopy (AFM) in different environmental conditions. The AFM results show that the two strains have considerable difference in the surface morphology. At the same time after rehydration both strains show the loss of the topographic features and increase in lateral and vertical dimensions. Results obtained in different AFM modes (contact, tapping, MAC) were compared. Imaging in culture medium was applied for direct observation of the surface degradation effect of lysozyme. The treatment of the cells with the enzyme in the culture medium lead to the loss of surface rigidity and eventually to dramatic changes of the bacteria shape.

Original languageEnglish (US)
Pages (from-to)121-128
Number of pages8
JournalUltramicroscopy
Volume86
Issue number1-2
DOIs
StatePublished - Feb 6 2001

Fingerprint

bacteria
Atomic force microscopy
culture media
Bacteria
atomic force microscopy
Culture Media
Enzymes
lysozyme
Muramidase
Escherichia
cells
Cell culture
rigidity
Rigidity
Escherichia coli
Surface morphology
enzymes
Cells
degradation
Imaging techniques

Keywords

  • Atomic force microscopy
  • Bacteria
  • Lysozyme
  • Surface morphology

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Instrumentation

Cite this

Bolshakova, A. V., Kiselyova, O. I., Filonov, A. S., Frolova, O. Y., Lyubchenko, Y. L., & Yaminsky, I. V. (2001). Comparative studies of bacteria with an atomic force microscopy operating in different modes. Ultramicroscopy, 86(1-2), 121-128. https://doi.org/10.1016/S0304-3991(00)00075-9

Comparative studies of bacteria with an atomic force microscopy operating in different modes. / Bolshakova, A. V.; Kiselyova, O. I.; Filonov, A. S.; Frolova, O. Yu; Lyubchenko, Y. L.; Yaminsky, I. V.

In: Ultramicroscopy, Vol. 86, No. 1-2, 06.02.2001, p. 121-128.

Research output: Contribution to journalArticle

Bolshakova, AV, Kiselyova, OI, Filonov, AS, Frolova, OY, Lyubchenko, YL & Yaminsky, IV 2001, 'Comparative studies of bacteria with an atomic force microscopy operating in different modes', Ultramicroscopy, vol. 86, no. 1-2, pp. 121-128. https://doi.org/10.1016/S0304-3991(00)00075-9
Bolshakova, A. V. ; Kiselyova, O. I. ; Filonov, A. S. ; Frolova, O. Yu ; Lyubchenko, Y. L. ; Yaminsky, I. V. / Comparative studies of bacteria with an atomic force microscopy operating in different modes. In: Ultramicroscopy. 2001 ; Vol. 86, No. 1-2. pp. 121-128.
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