Comparative properties of bacteriophage phi6 and phi6 nucleocapsid

J. V. Etten, L. Lane, C. Gonzalez, J. Partridge, A. Vidaver

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

Nonionic detergent treatments released a nucleocapsid from the enveloped bacteriophage phi 6. The nucleocapsid sedimented at nearly the same rate as the whole phage in sucrose density gradients, but the buoyant density in Cs2SO4 changed from 1.22g/cm3 for the whole phage to 1.33g/cm3 for the nucleocapsid. The detergent completely removed the lipid and 5 of the 10 proteins from the phage. Surface labeling of the phage and nucleocapsid with 125I revealed that protein P3 was on the outer surface of the whole phage and P8 was on the surface of the nucleocapsid. Both the phage and the nucleocapsid were stable between pH 6.0 and 9.5. Low concentrations of EDTA (10-4M) dissociated the nucleocapsid but had no effect on the whole phage. The nucleocapsid contained all three double stranded RNA segments, as well as RNA polymerase activity.

Original languageEnglish (US)
Pages (from-to)652-658
Number of pages7
JournalJournal of virology
Volume18
Issue number2
StatePublished - Dec 1 1976

Fingerprint

Nucleocapsid
nucleocapsid
bacteriophages
Bacteriophages
Bacteriophage phi 6
detergents
Detergents
Double-Stranded RNA
double-stranded RNA
DNA-Directed RNA Polymerases
DNA-directed RNA polymerase
Edetic Acid
Sucrose
Proteins
proteins
sucrose
Lipids
lipids

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Etten, J. V., Lane, L., Gonzalez, C., Partridge, J., & Vidaver, A. (1976). Comparative properties of bacteriophage phi6 and phi6 nucleocapsid. Journal of virology, 18(2), 652-658.

Comparative properties of bacteriophage phi6 and phi6 nucleocapsid. / Etten, J. V.; Lane, L.; Gonzalez, C.; Partridge, J.; Vidaver, A.

In: Journal of virology, Vol. 18, No. 2, 01.12.1976, p. 652-658.

Research output: Contribution to journalArticle

Etten, JV, Lane, L, Gonzalez, C, Partridge, J & Vidaver, A 1976, 'Comparative properties of bacteriophage phi6 and phi6 nucleocapsid', Journal of virology, vol. 18, no. 2, pp. 652-658.
Etten JV, Lane L, Gonzalez C, Partridge J, Vidaver A. Comparative properties of bacteriophage phi6 and phi6 nucleocapsid. Journal of virology. 1976 Dec 1;18(2):652-658.
Etten, J. V. ; Lane, L. ; Gonzalez, C. ; Partridge, J. ; Vidaver, A. / Comparative properties of bacteriophage phi6 and phi6 nucleocapsid. In: Journal of virology. 1976 ; Vol. 18, No. 2. pp. 652-658.
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