Comparative pathogenicity of selected bovine viral diarrhea virus isolates in gnotobiotic lambs.

C. J. Jewett, Clayton L Kelling, M. L. Frey, Alan R Doster

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Abstract

The infectivity and pathogenicity of selected bovine viral diarrhea virus (BVDV) isolates were determined in gnotobiotic, colostrum-deprived neonatal lambs. Five-day-old cesarean-derived gnotobiotic lambs were exposed to 1 of 10 BVDV isolates via aerosol suspension. These isolates were from tissues or secretions of calves or lambs affected with respiratory tract disease, weak neonatal calves, aborted bovine fetuses, or reference Singer or Draper BVDV. The pathogenicity of each isolate, relative to the others, was evaluated in lambs by measurement of the neutralizing antibody response, virus isolation from nasal secretions or tissues, and postmortem lesions. The BVDV isolates varied in their infectivity and pathogenicity. Singer, the cytopathic reference strain, was the most lymphotrophic isolate and stimulated the greatest neutralizing antibody response. Encephalitis was the most consistent lesion observed and was used as the final determinant of relative pathogenicity of the viruses. The most neuropathogenic isolates were the 2 viruses originating from lambs affected with respiratory tract disease, the 2 weak neonatal calf isolates, and 1 isolate from an aborted bovine fetus. The least pathogenic isolates were the 2 reference isolates, Draper and Singer; the 2 mucosal disease isolates; and 1 isolate originating from an aborted bovine fetus.

Original languageEnglish (US)
Pages (from-to)1640-1644
Number of pages5
JournalAmerican journal of veterinary research
Volume51
Issue number10
StatePublished - Oct 1 1990

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Germ-Free Life
Bovine Viral Diarrhea Viruses
Bovine viral diarrhea virus
Aborted Fetus
Virulence
lambs
pathogenicity
Respiratory Tract Diseases
Viruses
Neutralizing Antibodies
Antibody Formation
fetus
Bovine viral diarrhea virus 1
calves
neutralizing antibodies
lesions (animal)
viruses
respiratory tract diseases
Colostrum
cattle

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Comparative pathogenicity of selected bovine viral diarrhea virus isolates in gnotobiotic lambs. / Jewett, C. J.; Kelling, Clayton L; Frey, M. L.; Doster, Alan R.

In: American journal of veterinary research, Vol. 51, No. 10, 01.10.1990, p. 1640-1644.

Research output: Contribution to journalArticle

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