Comparative kinetic modeling of growth and molecular hydrogen overproduction by engineered strains of Thermotoga maritima

Raghuveer Singh, Rahul Tevatia, Derrick White, Yaşar Demirel, Paul Blum

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Thermotoga maritima is an anaerobic hyperthermophilic bacterium known for its high amounts of hydrogen (H 2 ) production. In the current study, the kinetic modeling was applied on the engineered strains of T. maritima that surpassed the natural H 2 production limit. The study generated a kinetic model explaining H 2 overproduction and predicted a continuous fermentation system. A Leudking-Piret equation-based model predicted that H 2 production by Tma200 (0.217 mol-H 2 g −1 -biomass) and Tma100 (0.147 mol-H 2 g −1 -biomass) were higher than wild type (0.096 mol-H 2 g −1 -biomass) with reduced rates of maltose utilization. Sensitivity analysis confirmed satisfactory fitting of the experimental data. The slow growth rates of Tma200 (0.550 h −1 ) and Tma100 (0.495 h −1 ) are compared with the wild type (0.663 h −1 ). A higher maintenance energy along with growth and non-growth H 2 coefficients corroborate the higher H 2 productivity of the engineered strains. The modeled data established a continuous fermentation system for the sustainable H 2 production.

Original languageEnglish (US)
Pages (from-to)7125-7136
Number of pages12
JournalInternational Journal of Hydrogen Energy
Volume44
Issue number14
DOIs
StatePublished - Mar 15 2019

Fingerprint

biomass
Hydrogen
fermentation
Biomass
Kinetics
kinetics
hydrogen
Fermentation
Maltose
sensitivity analysis
productivity
bacteria
Sensitivity analysis
maintenance
Bacteria
Productivity
coefficients
energy

Keywords

  • Bio hydrogen beyond Thauer limit
  • Biofuel
  • Continuous stirred tank reactor
  • Dark fermentation
  • Kinetic modeling
  • Natural gas phase out

ASJC Scopus subject areas

  • Renewable Energy, Sustainability and the Environment
  • Fuel Technology
  • Condensed Matter Physics
  • Energy Engineering and Power Technology

Cite this

Comparative kinetic modeling of growth and molecular hydrogen overproduction by engineered strains of Thermotoga maritima. / Singh, Raghuveer; Tevatia, Rahul; White, Derrick; Demirel, Yaşar; Blum, Paul.

In: International Journal of Hydrogen Energy, Vol. 44, No. 14, 15.03.2019, p. 7125-7136.

Research output: Contribution to journalArticle

Singh, Raghuveer ; Tevatia, Rahul ; White, Derrick ; Demirel, Yaşar ; Blum, Paul. / Comparative kinetic modeling of growth and molecular hydrogen overproduction by engineered strains of Thermotoga maritima. In: International Journal of Hydrogen Energy. 2019 ; Vol. 44, No. 14. pp. 7125-7136.
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