Comoviruses and enteroviruses share a T cell epitope

Melinda A. Beck, Steven Tracy, Beth Ann Coller, Nora M. Chapman, Günter Hufnagel, John E. Johnson, George Lomonossoff

Research output: Contribution to journalArticle

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Abstract

An in vitro murine T cell proliferation assay was used to determine whether an antigenic epitope(s) recognized by enterovirus-immune T cells is held in common between plant comoviruses and human enteroviruses. Splenocytes isolated from C3H/HeJ mice infected with coxsackievirus B3 (CVB3) proliferated in vitro not only against a variety of enterovirus (CVB2, CVB3, CVB6, CVA16, PV1) antigens, but against comovirus (CPMV, BPMV) antigens as well. Splenocytes from mice inoculated with bean pod mottle virus (BPMV) also proliferated in response to comoviral and enteroviral antigens in vitro. However, if the viral inocula were highly purified prior to inoculation, then the splenocyte response was generated only against the group used to inoculate, suggesting that the epitope shared between the comoviruses and the enteroviruses resided in the nonstructural region. B (nonstructural) and M (structural) genomic segments of CPMV were translated in rabbit reticulocyte lysates and used as in vitro antigens. Splenocytes from mice inoculated with live CVB3 proliferated in response to the B-RNA-encoded but not the M-RNA-encoded polypeptides, confirming the nonstructural coding region location of the common epitope. Comparison of predicted amino acid sequences in the nonstructural coding regions of the comoviruses and picornaviruses suggested a potentially immunogenic linear epitope in protein 2C. The consensus peptide LEEKGI was synthezized and shown to be immunogenic for both BPMV- and CVB3-immune splenocytes.b.

Original languageEnglish (US)
Pages (from-to)238-246
Number of pages9
JournalVirology
Volume186
Issue number1
DOIs
StatePublished - Jan 1992

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Comovirus
T-Lymphocyte Epitopes
Enterovirus
Epitopes
Antigens
Viruses
RNA
Picornaviridae
T-Lymphocytes
Peptides
Inbred C3H Mouse
Reticulocytes
Amino Acid Sequence
Cell Proliferation
Rabbits

ASJC Scopus subject areas

  • Virology

Cite this

Beck, M. A., Tracy, S., Coller, B. A., Chapman, N. M., Hufnagel, G., Johnson, J. E., & Lomonossoff, G. (1992). Comoviruses and enteroviruses share a T cell epitope. Virology, 186(1), 238-246. https://doi.org/10.1016/0042-6822(92)90078-4

Comoviruses and enteroviruses share a T cell epitope. / Beck, Melinda A.; Tracy, Steven; Coller, Beth Ann; Chapman, Nora M.; Hufnagel, Günter; Johnson, John E.; Lomonossoff, George.

In: Virology, Vol. 186, No. 1, 01.1992, p. 238-246.

Research output: Contribution to journalArticle

Beck, MA, Tracy, S, Coller, BA, Chapman, NM, Hufnagel, G, Johnson, JE & Lomonossoff, G 1992, 'Comoviruses and enteroviruses share a T cell epitope', Virology, vol. 186, no. 1, pp. 238-246. https://doi.org/10.1016/0042-6822(92)90078-4
Beck MA, Tracy S, Coller BA, Chapman NM, Hufnagel G, Johnson JE et al. Comoviruses and enteroviruses share a T cell epitope. Virology. 1992 Jan;186(1):238-246. https://doi.org/10.1016/0042-6822(92)90078-4
Beck, Melinda A. ; Tracy, Steven ; Coller, Beth Ann ; Chapman, Nora M. ; Hufnagel, Günter ; Johnson, John E. ; Lomonossoff, George. / Comoviruses and enteroviruses share a T cell epitope. In: Virology. 1992 ; Vol. 186, No. 1. pp. 238-246.
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