Combined Collagen-Induced Arthritis and Organic Dust-Induced Airway Inflammation to Model Inflammatory Lung Disease in Rheumatoid Arthritis

Jill A. Poole, Geoffrey M. Thiele, Katherine Janike, Amy J. Nelson, Michael J. Duryee, Kathryn Rentfro, Bryant R. England, Debra J. Romberger, Joseph M. Carrington, Dong Wang, Benjamin J. Swanson, Lynell W. Klassen, Ted R. Mikuls

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Rheumatoid arthritis (RA) is characterized by extra-articular involvement including lung disease, yet the mechanisms linking the two conditions are poorly understood. The collagen-induced arthritis (CIA) model was combined with the organic dust extract (ODE) airway inflammatory model to assess bone/joint–lung inflammatory outcomes. DBA/1J mice were intranasally treated with saline or ODE daily for 5 weeks. CIA was induced on days 1 and 21. Treatment groups included sham (saline injection/saline inhalation), CIA (CIA/saline), ODE (saline/ODE), and CIA + ODE (CIA/ODE). Arthritis inflammatory scores, bones, bronchoalveolar lavage fluid, lung tissues, and serum were assessed. In DBA/1J male mice, arthritis was increased in CIA + ODE > CIA > ODE versus sham. Micro-computed tomography (µCT) demonstrated that loss of BMD and volume and deterioration of bone microarchitecture was greatest in CIA + ODE. However, ODE-induced airway neutrophil influx and inflammatory cytokine/chemokine levels in lavage fluids were increased in ODE > CIA + ODE versus sham. Activated lung CD11c+CD11b+ macrophages were increased in ODE > CIA + ODE > CIA pattern, whereas lung hyaluronan, fibronectin, and amphiregulin levels were greatest in CIA + ODE. Serum autoantibody and inflammatory marker concentrations varied among experimental groups. Compared with male mice, female mice showed less articular and pulmonary disease. The interaction of inhalation-induced airway inflammation and arthritis induction resulted in compartmentalized responses with the greatest degree of arthritis and bone loss in male mice with combined exposures. Data also support suppression of the lung inflammatory response, but increases in extracellular matrix protein deposition/interstitial disease in the setting of arthritis. This coexposure model could be exploited to better understand and treat RA–lung disease.

Original languageEnglish (US)
Pages (from-to)1733-1743
Number of pages11
JournalJournal of Bone and Mineral Research
Volume34
Issue number9
DOIs
StatePublished - Sep 1 2019

Fingerprint

Experimental Arthritis
Dust
Lung Diseases
Rheumatoid Arthritis
Inflammation
Arthritis
Bone and Bones
Lung
Inhalation
Joints
Inbred DBA Mouse
Extracellular Matrix Proteins
Therapeutic Irrigation
Bronchoalveolar Lavage Fluid
Hyaluronic Acid
Serum
Fibronectins
Chemokines
Autoantibodies
Neutrophils

Keywords

  • AUTOIMMUNITY
  • COEXPOSURE
  • INFLAMMATION
  • INTERSTITIAL LUNG DISEASE
  • RHEUMATOID ARTHRITIS

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Orthopedics and Sports Medicine

Cite this

Combined Collagen-Induced Arthritis and Organic Dust-Induced Airway Inflammation to Model Inflammatory Lung Disease in Rheumatoid Arthritis. / Poole, Jill A.; Thiele, Geoffrey M.; Janike, Katherine; Nelson, Amy J.; Duryee, Michael J.; Rentfro, Kathryn; England, Bryant R.; Romberger, Debra J.; Carrington, Joseph M.; Wang, Dong; Swanson, Benjamin J.; Klassen, Lynell W.; Mikuls, Ted R.

In: Journal of Bone and Mineral Research, Vol. 34, No. 9, 01.09.2019, p. 1733-1743.

Research output: Contribution to journalArticle

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abstract = "Rheumatoid arthritis (RA) is characterized by extra-articular involvement including lung disease, yet the mechanisms linking the two conditions are poorly understood. The collagen-induced arthritis (CIA) model was combined with the organic dust extract (ODE) airway inflammatory model to assess bone/joint–lung inflammatory outcomes. DBA/1J mice were intranasally treated with saline or ODE daily for 5 weeks. CIA was induced on days 1 and 21. Treatment groups included sham (saline injection/saline inhalation), CIA (CIA/saline), ODE (saline/ODE), and CIA + ODE (CIA/ODE). Arthritis inflammatory scores, bones, bronchoalveolar lavage fluid, lung tissues, and serum were assessed. In DBA/1J male mice, arthritis was increased in CIA + ODE > CIA > ODE versus sham. Micro-computed tomography (µCT) demonstrated that loss of BMD and volume and deterioration of bone microarchitecture was greatest in CIA + ODE. However, ODE-induced airway neutrophil influx and inflammatory cytokine/chemokine levels in lavage fluids were increased in ODE > CIA + ODE versus sham. Activated lung CD11c+CD11b+ macrophages were increased in ODE > CIA + ODE > CIA pattern, whereas lung hyaluronan, fibronectin, and amphiregulin levels were greatest in CIA + ODE. Serum autoantibody and inflammatory marker concentrations varied among experimental groups. Compared with male mice, female mice showed less articular and pulmonary disease. The interaction of inhalation-induced airway inflammation and arthritis induction resulted in compartmentalized responses with the greatest degree of arthritis and bone loss in male mice with combined exposures. Data also support suppression of the lung inflammatory response, but increases in extracellular matrix protein deposition/interstitial disease in the setting of arthritis. This coexposure model could be exploited to better understand and treat RA–lung disease.",
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AU - Janike, Katherine

AU - Nelson, Amy J.

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AU - England, Bryant R.

AU - Romberger, Debra J.

AU - Carrington, Joseph M.

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