Combinatorial evaluation of in vivo distribution of polyanhydride particle-based platforms for vaccine delivery

Latrisha K. Petersen, Lucas Huntimer, Katharine Walz, Amanda Ramer-Tait, Michael J. Wannemuehler, Balaji Narasimhan

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Several challenges are associated with current vaccine strategies, including repeated immunizations, poor patient compliance, and limited approved routes for delivery, which may hinder induction of protective immunity. Thus, there is a need for new vaccine adjuvants capable of multi-route administration and prolonged antigen release at the site of administration by providing a depot within tissue. In this work, we designed a combinatorial platform to investigate the in vivo distribution, depot effect, and localized persistence of polyanhydride nanoparticles as a function of nanoparticle chemistry and administration route. Our observations indicated that the route of administration differentially affected tissue residence times. All nanoparticles rapidly dispersed when delivered intranasally but provided a depot when administered parenterally. When amphiphilic and hydrophobic nanoparticles were administered intranasally, they persisted within lung tissue. These results provide insights into the chemistry and route-dependent distribution and tissue-specific association of polyanhydride nanoparticle-based vaccine adjuvants.

Original languageEnglish (US)
Pages (from-to)2213-2225
Number of pages13
JournalInternational journal of nanomedicine
Volume8
DOIs
StatePublished - Jun 17 2013

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Keywords

  • Combinatorial
  • Distribution
  • Live animal imaging
  • Nanoparticle
  • Polyanhydride

ASJC Scopus subject areas

  • Biophysics
  • Bioengineering
  • Biomaterials
  • Pharmaceutical Science
  • Drug Discovery
  • Organic Chemistry

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