Combinatorial energy transfer between an end-capped conjugated polyelectrolyte and chromophore-labeled PNA for strand-specific DNA detection

Shudipto Konika Dishari, Kan Yi Pu, Bin Liu

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We report a macromolecular end-capping approach to improve the detection sensitivity of cationic conjugated polymer (CCP) based DNA detection. A phenylethynyl anthracene (PEA) end-capped cationic polyfluorene (PF) derivative (P1) is synthesized via Suzuki coupling. Due to efficient fluorescence resonance energy transfer (FRET) from the polymer backbone to the endcapper PEA units, the polymer (P1) fluorescence is dominated by the emission from PEA even in dilute aqueous solution. P1 emission has a better spectral overlap with fluorescein (F1) absorption compared to that for uncapped PF (P2). In addition, the intra and intermolecular energy transfer for P1 is more efficient in the presence of DNA due to complexation-induced polymer aggregation. These impart a combinatorial FRET between P1 and an Fl-labeled probe which is more efficient than that between P2 and the same probe. P1 thus offers a better DNA detection sensitivity relative to P2 and opens up new opportunities to improve the performance of CCP based biosensors involving FRET.

Original languageEnglish (US)
Pages (from-to)1645-1650
Number of pages6
JournalMacromolecular Rapid Communications
Volume30
Issue number19
DOIs
StatePublished - Oct 1 2009

Fingerprint

Anthracene
Chromophores
Polyelectrolytes
Energy transfer
Polymers
DNA
Conjugated polymers
Complexation
Fluorescein
Biosensors
Agglomeration
Fluorescence
Derivatives
Fluorescence Resonance Energy Transfer
anthracene

Keywords

  • Conjugated polymers
  • Fluorescence
  • Peptides
  • Sensors
  • Synthesis

ASJC Scopus subject areas

  • Organic Chemistry
  • Polymers and Plastics
  • Materials Chemistry

Cite this

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title = "Combinatorial energy transfer between an end-capped conjugated polyelectrolyte and chromophore-labeled PNA for strand-specific DNA detection",
abstract = "We report a macromolecular end-capping approach to improve the detection sensitivity of cationic conjugated polymer (CCP) based DNA detection. A phenylethynyl anthracene (PEA) end-capped cationic polyfluorene (PF) derivative (P1) is synthesized via Suzuki coupling. Due to efficient fluorescence resonance energy transfer (FRET) from the polymer backbone to the endcapper PEA units, the polymer (P1) fluorescence is dominated by the emission from PEA even in dilute aqueous solution. P1 emission has a better spectral overlap with fluorescein (F1) absorption compared to that for uncapped PF (P2). In addition, the intra and intermolecular energy transfer for P1 is more efficient in the presence of DNA due to complexation-induced polymer aggregation. These impart a combinatorial FRET between P1 and an Fl-labeled probe which is more efficient than that between P2 and the same probe. P1 thus offers a better DNA detection sensitivity relative to P2 and opens up new opportunities to improve the performance of CCP based biosensors involving FRET.",
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author = "Dishari, {Shudipto Konika} and Pu, {Kan Yi} and Bin Liu",
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AU - Dishari, Shudipto Konika

AU - Pu, Kan Yi

AU - Liu, Bin

PY - 2009/10/1

Y1 - 2009/10/1

N2 - We report a macromolecular end-capping approach to improve the detection sensitivity of cationic conjugated polymer (CCP) based DNA detection. A phenylethynyl anthracene (PEA) end-capped cationic polyfluorene (PF) derivative (P1) is synthesized via Suzuki coupling. Due to efficient fluorescence resonance energy transfer (FRET) from the polymer backbone to the endcapper PEA units, the polymer (P1) fluorescence is dominated by the emission from PEA even in dilute aqueous solution. P1 emission has a better spectral overlap with fluorescein (F1) absorption compared to that for uncapped PF (P2). In addition, the intra and intermolecular energy transfer for P1 is more efficient in the presence of DNA due to complexation-induced polymer aggregation. These impart a combinatorial FRET between P1 and an Fl-labeled probe which is more efficient than that between P2 and the same probe. P1 thus offers a better DNA detection sensitivity relative to P2 and opens up new opportunities to improve the performance of CCP based biosensors involving FRET.

AB - We report a macromolecular end-capping approach to improve the detection sensitivity of cationic conjugated polymer (CCP) based DNA detection. A phenylethynyl anthracene (PEA) end-capped cationic polyfluorene (PF) derivative (P1) is synthesized via Suzuki coupling. Due to efficient fluorescence resonance energy transfer (FRET) from the polymer backbone to the endcapper PEA units, the polymer (P1) fluorescence is dominated by the emission from PEA even in dilute aqueous solution. P1 emission has a better spectral overlap with fluorescein (F1) absorption compared to that for uncapped PF (P2). In addition, the intra and intermolecular energy transfer for P1 is more efficient in the presence of DNA due to complexation-induced polymer aggregation. These impart a combinatorial FRET between P1 and an Fl-labeled probe which is more efficient than that between P2 and the same probe. P1 thus offers a better DNA detection sensitivity relative to P2 and opens up new opportunities to improve the performance of CCP based biosensors involving FRET.

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KW - Fluorescence

KW - Peptides

KW - Sensors

KW - Synthesis

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