Coherent anti-stokes Raman scattering and spontaneous Raman spectroscopy and microscopy of microalgae with nitrogen depletion

X. N. He, J. Allen, P. N. Black, T. Baldacchini, X. Huang, H. Huang, L. Jiang, Y. F. Lu

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Microalgae are extensively researched as potential feedstocks for biofuel production. Energy-rich compounds in microalgae, such as lipids, require efficient characterization techniques to investigate the metabolic pathways and the environmental factors influencing their accumulation. The model green alga Coccomyxa accumulates significant amounts of triacylglycerols (TAGs) under nitrogen depletion (N-depletion). To monitor the growth of TAGs (lipid) in microalgal cells, a study of microalgal cells (Coccomyxa sp. C169) using both spontaneous Raman and coherent anti- Stokes Raman scattering (CARS) spectroscopy and microscopy were carried out. Spontaneous Raman spectroscopy was conducted to analyze the components in the algal cells, while CARS was carried out to monitor the distribution of lipid droplets in the cells. Raman signals of carotenoid are greater in control microalgae compared to N-depleted cells. Raman signals of lipid droplets appear after N-depletion and its distribution can be clearly observed in the CARS microscopy. Both spontaneous Raman spectroscopy and CARS microscopy were found to be suitable analysis tools for microalgae.

Original languageEnglish (US)
Pages (from-to)2896-2906
Number of pages11
JournalBiomedical Optics Express
Volume3
Issue number11
DOIs
StatePublished - Nov 1 2012

Fingerprint

Microalgae
Raman Spectrum Analysis
Microscopy
depletion
Nitrogen
Raman spectroscopy
lipids
Raman spectra
microscopy
nitrogen
cells
carotenoids
Triglycerides
algae
Lipids
Chlorophyta
Biofuels
Carotenoids
Metabolic Networks and Pathways
spectroscopy

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

Cite this

Coherent anti-stokes Raman scattering and spontaneous Raman spectroscopy and microscopy of microalgae with nitrogen depletion. / He, X. N.; Allen, J.; Black, P. N.; Baldacchini, T.; Huang, X.; Huang, H.; Jiang, L.; Lu, Y. F.

In: Biomedical Optics Express, Vol. 3, No. 11, 01.11.2012, p. 2896-2906.

Research output: Contribution to journalArticle

He, X. N. ; Allen, J. ; Black, P. N. ; Baldacchini, T. ; Huang, X. ; Huang, H. ; Jiang, L. ; Lu, Y. F. / Coherent anti-stokes Raman scattering and spontaneous Raman spectroscopy and microscopy of microalgae with nitrogen depletion. In: Biomedical Optics Express. 2012 ; Vol. 3, No. 11. pp. 2896-2906.
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