Cocrystallization studies of full-length recombinant butyrylcholinesterase (BChE) with cocaine

Oluwatoyin Ajibola Asojo, Oluyomi Adebola Asojo, Michelle N. Ngamelue, Kohei Homma, Oksana Lockridge

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Human butyrylcholinesterase (BChE; EC 3.1.1.8) is a 340 kDa tetrameric glycoprotein that is present in human serum at about 5 mg l-1 and has well documented therapeutic effects on cocaine toxicity. BChE holds promise as a therapeutic that reduces and finally eliminates the rewarding effects of cocaine, thus weaning an addict from the drug. There have been extensive computational studies of cocaine hydrolysis by BChE. Since there are no reported structures of BChE with cocaine or any of the hydrolysis products, full-length monomeric recombinant wild-type BChE was cocrystallized with cocaine. The refined 3 Å resolution structure appears to retain the hydrolysis product benzoic acid in sufficient proximity to form a hydrogen bond to the active-site Ser198.

Original languageEnglish (US)
Pages (from-to)434-437
Number of pages4
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume67
Issue number4
DOIs
Publication statusPublished - Apr 1 2011

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Keywords

  • BChE
  • cocaine hydrolysis
  • recombinant butyrylcholinesterase

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

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