Coating electrospun poly(ε-caprolactone) fibers with gelatin and calcium phosphate and their use as biomimetic scaffolds for bone tissue engineering

Xiaoran Li, Jingwei Xie, Xiaoyan Yuan, Younan Xia

Research output: Contribution to journalArticle

175 Citations (Scopus)

Abstract

Electrospinning was employed to fabricate fibrous scaffolds of poly(ε-caprolactone) in the form of nonwoven mats. The surfaces of the fibers were then coated with gelatin through layer-by-layer self-assembly, followed by functionalization with a uniform coating of bonelike calcium phosphate by mineralization in the 10 times concentrated simulated body fluid for 2 h. Transmission electron microscopy, water contact angle, and scanning electron microscopy measurements confirmed the presence of gelatin and calcium phosphate coating layers, and X-ray diffraction results suggested that the deposited mineral phase was a mixture of dicalcium phosphate dehydrate (a precursor to apatite) and apatite. It was also demonstrated that the incorporation of gelatin promoted nucleation and growth of calcium phosphate. The porous scaffolds could mimic the structure, composition, and biological function of bone extracellular matrix. It was found that the preosteoblastic MC3T3-E1 cells attached, spread, and proliferated well with a flat morphology on the mineralized scaffolds. The proliferation rate of the cells on the mineralized scaffolds was significantly higher (by 1.9-fold) than that on the pristine fibrous scaffolds after culture for 7 days. These results indicated that the hybrid system containing poly(ε-caprolactone), gelatin, and calcium phosphate could serve as a new class of biomimetic scaffolds for bone tissue engineering.

Original languageEnglish (US)
Pages (from-to)14145-14150
Number of pages6
JournalLangmuir
Volume24
Issue number24
DOIs
StatePublished - Dec 16 2008
Externally publishedYes

Fingerprint

calcium phosphates
tissue engineering
biomimetics
gelatins
Calcium phosphate
Biomimetics
Gelatin
Scaffolds (biology)
Tissue engineering
Scaffolds
bones
phosphates
Bone
coatings
Apatites
Coatings
fibers
Fibers
Apatite
apatites

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

Cite this

Coating electrospun poly(ε-caprolactone) fibers with gelatin and calcium phosphate and their use as biomimetic scaffolds for bone tissue engineering. / Li, Xiaoran; Xie, Jingwei; Yuan, Xiaoyan; Xia, Younan.

In: Langmuir, Vol. 24, No. 24, 16.12.2008, p. 14145-14150.

Research output: Contribution to journalArticle

@article{0c3939ca8e9e4b4fb1e4e749c511148c,
title = "Coating electrospun poly(ε-caprolactone) fibers with gelatin and calcium phosphate and their use as biomimetic scaffolds for bone tissue engineering",
abstract = "Electrospinning was employed to fabricate fibrous scaffolds of poly(ε-caprolactone) in the form of nonwoven mats. The surfaces of the fibers were then coated with gelatin through layer-by-layer self-assembly, followed by functionalization with a uniform coating of bonelike calcium phosphate by mineralization in the 10 times concentrated simulated body fluid for 2 h. Transmission electron microscopy, water contact angle, and scanning electron microscopy measurements confirmed the presence of gelatin and calcium phosphate coating layers, and X-ray diffraction results suggested that the deposited mineral phase was a mixture of dicalcium phosphate dehydrate (a precursor to apatite) and apatite. It was also demonstrated that the incorporation of gelatin promoted nucleation and growth of calcium phosphate. The porous scaffolds could mimic the structure, composition, and biological function of bone extracellular matrix. It was found that the preosteoblastic MC3T3-E1 cells attached, spread, and proliferated well with a flat morphology on the mineralized scaffolds. The proliferation rate of the cells on the mineralized scaffolds was significantly higher (by 1.9-fold) than that on the pristine fibrous scaffolds after culture for 7 days. These results indicated that the hybrid system containing poly(ε-caprolactone), gelatin, and calcium phosphate could serve as a new class of biomimetic scaffolds for bone tissue engineering.",
author = "Xiaoran Li and Jingwei Xie and Xiaoyan Yuan and Younan Xia",
year = "2008",
month = "12",
day = "16",
doi = "10.1021/la802984a",
language = "English (US)",
volume = "24",
pages = "14145--14150",
journal = "Langmuir",
issn = "0743-7463",
publisher = "American Chemical Society",
number = "24",

}

TY - JOUR

T1 - Coating electrospun poly(ε-caprolactone) fibers with gelatin and calcium phosphate and their use as biomimetic scaffolds for bone tissue engineering

AU - Li, Xiaoran

AU - Xie, Jingwei

AU - Yuan, Xiaoyan

AU - Xia, Younan

PY - 2008/12/16

Y1 - 2008/12/16

N2 - Electrospinning was employed to fabricate fibrous scaffolds of poly(ε-caprolactone) in the form of nonwoven mats. The surfaces of the fibers were then coated with gelatin through layer-by-layer self-assembly, followed by functionalization with a uniform coating of bonelike calcium phosphate by mineralization in the 10 times concentrated simulated body fluid for 2 h. Transmission electron microscopy, water contact angle, and scanning electron microscopy measurements confirmed the presence of gelatin and calcium phosphate coating layers, and X-ray diffraction results suggested that the deposited mineral phase was a mixture of dicalcium phosphate dehydrate (a precursor to apatite) and apatite. It was also demonstrated that the incorporation of gelatin promoted nucleation and growth of calcium phosphate. The porous scaffolds could mimic the structure, composition, and biological function of bone extracellular matrix. It was found that the preosteoblastic MC3T3-E1 cells attached, spread, and proliferated well with a flat morphology on the mineralized scaffolds. The proliferation rate of the cells on the mineralized scaffolds was significantly higher (by 1.9-fold) than that on the pristine fibrous scaffolds after culture for 7 days. These results indicated that the hybrid system containing poly(ε-caprolactone), gelatin, and calcium phosphate could serve as a new class of biomimetic scaffolds for bone tissue engineering.

AB - Electrospinning was employed to fabricate fibrous scaffolds of poly(ε-caprolactone) in the form of nonwoven mats. The surfaces of the fibers were then coated with gelatin through layer-by-layer self-assembly, followed by functionalization with a uniform coating of bonelike calcium phosphate by mineralization in the 10 times concentrated simulated body fluid for 2 h. Transmission electron microscopy, water contact angle, and scanning electron microscopy measurements confirmed the presence of gelatin and calcium phosphate coating layers, and X-ray diffraction results suggested that the deposited mineral phase was a mixture of dicalcium phosphate dehydrate (a precursor to apatite) and apatite. It was also demonstrated that the incorporation of gelatin promoted nucleation and growth of calcium phosphate. The porous scaffolds could mimic the structure, composition, and biological function of bone extracellular matrix. It was found that the preosteoblastic MC3T3-E1 cells attached, spread, and proliferated well with a flat morphology on the mineralized scaffolds. The proliferation rate of the cells on the mineralized scaffolds was significantly higher (by 1.9-fold) than that on the pristine fibrous scaffolds after culture for 7 days. These results indicated that the hybrid system containing poly(ε-caprolactone), gelatin, and calcium phosphate could serve as a new class of biomimetic scaffolds for bone tissue engineering.

UR - http://www.scopus.com/inward/record.url?scp=61849152866&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=61849152866&partnerID=8YFLogxK

U2 - 10.1021/la802984a

DO - 10.1021/la802984a

M3 - Article

VL - 24

SP - 14145

EP - 14150

JO - Langmuir

JF - Langmuir

SN - 0743-7463

IS - 24

ER -