Coated microneedles for drug delivery to the eye

Jason Jiang, Harvinder S. Gill, Deepta Ghate, Bernard E. McCarey, Samir R. Patel, Henry F. Edelhauser, Mark R. Prausnitz

Research output: Contribution to journalArticle

94 Citations (Scopus)

Abstract

PURPOSE. To test the hypothesis that coated microneedles can deliver drugs into the eye via intrascleral and intracorneal routes in a minimally invasive manner. METHODS. Solid metal microneedles measuring 500 to 750 μm in length were coated with model drugs, protein, and DNA; inserted into nonpreserved human cadaveric sclera; and imaged. Microneedles coated with sodium fluorescein were then inserted into rabbit cornea in vivo. After needle removal, fluorescein concentration in the anterior segment of the rabbit eye was measured for 24 hours. Similar experiments were performed using pilocarpine-coated microneedles, and the rabbit pupil size was monitored afterward. RESULTS. In vitro insertion tests showed that microneedles were mechanically strong enough to penetrate into human cadaveric sclera and that the drug coating rapidly dissolved off the needles within the scleral tissue within 30 seconds after insertion. In vivo delivery from fluorescein-coated microneedles showed that fluorescein concentrations in the anterior chamber were 60 times greater than those achieved by topical application without microneedles. Similarly, microneedle delivery of pilocarpine caused rapid and extensive rabbit pupil constriction. There were no measurable inflammatory responses caused by microneedle insertion. CONCLUSIONS. This study demonstrated for the first time that coated microneedles can deliver drugs into the eye via intrascleral and intracorneal routes. This minimally invasive approach may avoid the complications associated with intraocular injection and systemic administration.

Original languageEnglish (US)
Pages (from-to)4038-4043
Number of pages6
JournalInvestigative Ophthalmology and Visual Science
Volume48
Issue number9
DOIs
StatePublished - Sep 1 2007

Fingerprint

Fluorescein
Rabbits
Sclera
Pilocarpine
Pupil
Pharmaceutical Preparations
Needles
Intraocular Injections
Anterior Eye Segment
Anterior Chamber
Constriction
Cornea
Metals
DNA
Proteins

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Jiang, J., Gill, H. S., Ghate, D., McCarey, B. E., Patel, S. R., Edelhauser, H. F., & Prausnitz, M. R. (2007). Coated microneedles for drug delivery to the eye. Investigative Ophthalmology and Visual Science, 48(9), 4038-4043. https://doi.org/10.1167/iovs.07-0066

Coated microneedles for drug delivery to the eye. / Jiang, Jason; Gill, Harvinder S.; Ghate, Deepta; McCarey, Bernard E.; Patel, Samir R.; Edelhauser, Henry F.; Prausnitz, Mark R.

In: Investigative Ophthalmology and Visual Science, Vol. 48, No. 9, 01.09.2007, p. 4038-4043.

Research output: Contribution to journalArticle

Jiang, J, Gill, HS, Ghate, D, McCarey, BE, Patel, SR, Edelhauser, HF & Prausnitz, MR 2007, 'Coated microneedles for drug delivery to the eye', Investigative Ophthalmology and Visual Science, vol. 48, no. 9, pp. 4038-4043. https://doi.org/10.1167/iovs.07-0066
Jiang J, Gill HS, Ghate D, McCarey BE, Patel SR, Edelhauser HF et al. Coated microneedles for drug delivery to the eye. Investigative Ophthalmology and Visual Science. 2007 Sep 1;48(9):4038-4043. https://doi.org/10.1167/iovs.07-0066
Jiang, Jason ; Gill, Harvinder S. ; Ghate, Deepta ; McCarey, Bernard E. ; Patel, Samir R. ; Edelhauser, Henry F. ; Prausnitz, Mark R. / Coated microneedles for drug delivery to the eye. In: Investigative Ophthalmology and Visual Science. 2007 ; Vol. 48, No. 9. pp. 4038-4043.
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