Co-exposure to cigarette smoke and alcohol decreases airway epithelial cell cilia beating in a protein kinase Cε-dependent manner

Todd A Wyatt, Joseph Harold Sisson, Diane S. Allen-Gipson, Michael L. McCaskill, Jessica A. Boten, Jane M. Devasure, Kristina L Bailey, Jill A Poole

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Alcohol use disorders are associated with increased lung infections and exacerbations of chronic lung diseases. Whereas the effects of cigarette smoke are well recognized, the interplay of smoke and alcohol in modulating lung diseases is not clear. Because innate lung defense is mechanically maintained by airway cilia action and protein kinase C (PKC)-activating agents slow ciliary beat frequency (CBF), we hypothesized that the combination of smoke and alcohol would decrease CBF in a PKC-dependent manner. Primary ciliated bronchial epithelial cells were exposed to 5% cigarette smoke extract plus100 mmol/L ethanol for up to 24 hours and assayed for CBF and PKCε. Smoke and alcohol co-exposure activated PKCε by 1 hour and decreased both CBF and total number of beating cilia by 6 hours. A specific activator of PKCε, DCP-LA, slowed CBF after maximal PKCε activation. Interestingly, activation of PKCε by smoke and alcohol was only observed in ciliated cells, not basal bronchial epithelium. In precision-cut mouse lung slices treated with smoke and alcohol, PKCε activation preceded CBF slowing. Correspondingly, increased PKCε activity and cilia slowing were only observed in mice co-exposed to smoke and alcohol, regardless of the sequence of the combination exposure. No decreases in CBF were observed in PKCε knockout mice co-exposed to smoke and alcohol. These data identify PKCε as a key regulator of cilia slowing in response to combined smoke and alcohol-induced lung injury.

Original languageEnglish (US)
Pages (from-to)431-440
Number of pages10
JournalAmerican Journal of Pathology
Volume181
Issue number2
DOIs
StatePublished - Aug 1 2012

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Cilia
Smoke
Tobacco Products
Protein Kinase C
Epithelial Cells
Alcohols
Lung
Lung Diseases
Lung Injury
Knockout Mice
Chronic Disease
Ethanol
Epithelium

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Co-exposure to cigarette smoke and alcohol decreases airway epithelial cell cilia beating in a protein kinase Cε-dependent manner. / Wyatt, Todd A; Sisson, Joseph Harold; Allen-Gipson, Diane S.; McCaskill, Michael L.; Boten, Jessica A.; Devasure, Jane M.; Bailey, Kristina L; Poole, Jill A.

In: American Journal of Pathology, Vol. 181, No. 2, 01.08.2012, p. 431-440.

Research output: Contribution to journalArticle

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