Cloning of mtDNA fragments homologous to mitochondrial S2 plasmid-like DNA in maize

Csaba Koncz, Janos Sumegi, Andor Udvardy, Máté Racsmány, Dénes Dudits

Research output: Contribution to journalArticle

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Abstract

Mitochondria from S-type cytoplasmic male-sterile maize contain two small DNA species, S1 and S2, which are absent from other fertile and male-sterile cytoplasms. These species have been cloned in plasmid pBR322 by the homopolymer extension method. Probes made with these recombinant plasmids have been used to establish the homology between high molecular weight mitochondrial DNAs of fertile and male-sterile cytoplasms, and small mitochondrial plasmid-like molecules. Hybridization and mapping data show that S2 DNA copies are homologuous with sequences of the normal mitochondrial genome. A comparison of physical maps of different isolated mtDNA fragments indicates a heterogeneous arrangement of S2 sequences in the mtDNA population of normal fertile maize cytoplasm. The origin of this heterogeneity is discussed.

Original languageEnglish (US)
Pages (from-to)449-458
Number of pages10
JournalMGG Molecular & General Genetics
Volume183
Issue number3
DOIs
StatePublished - Nov 1 1981

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Mitochondrial DNA
Zea mays
Organism Cloning
Cytoplasm
Plasmids
DNA
Mitochondrial Genome
Mitochondria
Molecular Weight
Population

ASJC Scopus subject areas

  • Genetics

Cite this

Cloning of mtDNA fragments homologous to mitochondrial S2 plasmid-like DNA in maize. / Koncz, Csaba; Sumegi, Janos; Udvardy, Andor; Racsmány, Máté; Dudits, Dénes.

In: MGG Molecular & General Genetics, Vol. 183, No. 3, 01.11.1981, p. 449-458.

Research output: Contribution to journalArticle

Koncz, C, Sumegi, J, Udvardy, A, Racsmány, M & Dudits, D 1981, 'Cloning of mtDNA fragments homologous to mitochondrial S2 plasmid-like DNA in maize', MGG Molecular & General Genetics, vol. 183, no. 3, pp. 449-458. https://doi.org/10.1007/BF00268764
Koncz, Csaba ; Sumegi, Janos ; Udvardy, Andor ; Racsmány, Máté ; Dudits, Dénes. / Cloning of mtDNA fragments homologous to mitochondrial S2 plasmid-like DNA in maize. In: MGG Molecular & General Genetics. 1981 ; Vol. 183, No. 3. pp. 449-458.
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