Cloning of a galacturonic acid uptake gene from Erwinia chrysanthemi EC16

Thomas L. Freeman, Michael J.D. San Francisco

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A 3.4 kb fragment of Erwinia chrysanthemi EC16 DNA capable of complementing galacturonic acid uptake mutants (exuT) was identified and cloned into a multicopy vector. In E. chrysanthemi B374 exuT mutants, the cloned DNA provided for growth of the mutant strains on galacturonic acid by complementing the galacturonic acid uptake defect. Alkaline phosphatase (phoA) gene fusions with the cloned DNA suggested that most of the cloned DNA was necessary for complementation of exuT mutant strains. Using anti-alkaline phosphatase antibody, a hybrid ExuT-PhoA protein was localized to the membrane fraction of the bacterium.

Original languageEnglish (US)
Pages (from-to)101-106
Number of pages6
JournalFEMS Microbiology Letters
Volume118
Issue number1-2
DOIs
StatePublished - May 1 1994

Fingerprint

Pectobacterium chrysanthemi
Organism Cloning
DNA
Genes
Alkaline Phosphatase
Gene Fusion
Bacteria
Membranes
galacturonic acid
Antibodies
Growth
Proteins

Keywords

  • Erwinia chrysanthemi
  • Galacturonic acid transport
  • Pectate lyase
  • exuT

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Cloning of a galacturonic acid uptake gene from Erwinia chrysanthemi EC16. / Freeman, Thomas L.; San Francisco, Michael J.D.

In: FEMS Microbiology Letters, Vol. 118, No. 1-2, 01.05.1994, p. 101-106.

Research output: Contribution to journalArticle

Freeman, Thomas L. ; San Francisco, Michael J.D. / Cloning of a galacturonic acid uptake gene from Erwinia chrysanthemi EC16. In: FEMS Microbiology Letters. 1994 ; Vol. 118, No. 1-2. pp. 101-106.
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