Cloning, characterization, and genetics of the juvinile hormone esterase gene from Heliothis virescens

L. G. Harshman, V. K. Ward, J. K. Beetham, D. F. Grant, L. J. Grahan, C. A. Zraket, D. G. Heckel, B. D. Hammock

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

The gene for juvenile hormone esterase (JHE) was cloned from Heliothis virescens (Lepidoptera: Noctuidae). A genomic library was constructed from embryonic DNA and screened with a homologous N-terminal probe from the JHE cDNA. Five genomic clones were isolated and analyzed by dot blot hybridization using regions of the JHE cDNA as probes. Clone C hybridized to both 5′ and 3′ probes from the JHE cDNA, suggesting that clone C contains both ends of JHE gene. This was verified by sequencing the ends of the JHE gene from clone C using primers from both the 5′ and 3′ ends of the JHE cDNA. Additional sequencing and restriction mapping were used to characterized the gene. The gene is c. 8 kb long and contains four introns with consensus intron-exon junctions. One of the introns is relatively large (4 kb) and is situated near the extreme 5′ end of the gene. Genetic analysis of RFLP variation in interspecific and intraspecific crosses shows that the JHE locus is single-copy with no closely related paralogs and is autosomally encoded in Heliothis. Therefore the developmental pattern of expression of this gene and the previously documented sequence variation in cDNA clones is not explainable by reference to a JHE gene family with distinct structural loci for the different forms.

Original languageEnglish (US)
Pages (from-to)671-676
Number of pages6
JournalInsect Biochemistry and Molecular Biology
Volume24
Issue number7
DOIs
StatePublished - Jul 1994

Fingerprint

juvenile hormone esterase
Heliothis virescens
Cloning
Esterases
esterases
Organism Cloning
molecular cloning
Genes
hormones
Hormones
Complementary DNA
Clone Cells
genes
clones
Introns
introns
Heliothis
Genetics
Restriction Mapping
loci

Keywords

  • Heliothis virescens
  • Juvenile hormone esterase
  • Juvenile hormone esterase gene
  • Juvinile hormone

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Insect Science

Cite this

Harshman, L. G., Ward, V. K., Beetham, J. K., Grant, D. F., Grahan, L. J., Zraket, C. A., ... Hammock, B. D. (1994). Cloning, characterization, and genetics of the juvinile hormone esterase gene from Heliothis virescens. Insect Biochemistry and Molecular Biology, 24(7), 671-676. https://doi.org/10.1016/0965-1748(94)90054-X

Cloning, characterization, and genetics of the juvinile hormone esterase gene from Heliothis virescens. / Harshman, L. G.; Ward, V. K.; Beetham, J. K.; Grant, D. F.; Grahan, L. J.; Zraket, C. A.; Heckel, D. G.; Hammock, B. D.

In: Insect Biochemistry and Molecular Biology, Vol. 24, No. 7, 07.1994, p. 671-676.

Research output: Contribution to journalArticle

Harshman, LG, Ward, VK, Beetham, JK, Grant, DF, Grahan, LJ, Zraket, CA, Heckel, DG & Hammock, BD 1994, 'Cloning, characterization, and genetics of the juvinile hormone esterase gene from Heliothis virescens', Insect Biochemistry and Molecular Biology, vol. 24, no. 7, pp. 671-676. https://doi.org/10.1016/0965-1748(94)90054-X
Harshman, L. G. ; Ward, V. K. ; Beetham, J. K. ; Grant, D. F. ; Grahan, L. J. ; Zraket, C. A. ; Heckel, D. G. ; Hammock, B. D. / Cloning, characterization, and genetics of the juvinile hormone esterase gene from Heliothis virescens. In: Insect Biochemistry and Molecular Biology. 1994 ; Vol. 24, No. 7. pp. 671-676.
@article{20737407990b4b5d808a15085dafe514,
title = "Cloning, characterization, and genetics of the juvinile hormone esterase gene from Heliothis virescens",
abstract = "The gene for juvenile hormone esterase (JHE) was cloned from Heliothis virescens (Lepidoptera: Noctuidae). A genomic library was constructed from embryonic DNA and screened with a homologous N-terminal probe from the JHE cDNA. Five genomic clones were isolated and analyzed by dot blot hybridization using regions of the JHE cDNA as probes. Clone C hybridized to both 5′ and 3′ probes from the JHE cDNA, suggesting that clone C contains both ends of JHE gene. This was verified by sequencing the ends of the JHE gene from clone C using primers from both the 5′ and 3′ ends of the JHE cDNA. Additional sequencing and restriction mapping were used to characterized the gene. The gene is c. 8 kb long and contains four introns with consensus intron-exon junctions. One of the introns is relatively large (4 kb) and is situated near the extreme 5′ end of the gene. Genetic analysis of RFLP variation in interspecific and intraspecific crosses shows that the JHE locus is single-copy with no closely related paralogs and is autosomally encoded in Heliothis. Therefore the developmental pattern of expression of this gene and the previously documented sequence variation in cDNA clones is not explainable by reference to a JHE gene family with distinct structural loci for the different forms.",
keywords = "Heliothis virescens, Juvenile hormone esterase, Juvenile hormone esterase gene, Juvinile hormone",
author = "Harshman, {L. G.} and Ward, {V. K.} and Beetham, {J. K.} and Grant, {D. F.} and Grahan, {L. J.} and Zraket, {C. A.} and Heckel, {D. G.} and Hammock, {B. D.}",
year = "1994",
month = "7",
doi = "10.1016/0965-1748(94)90054-X",
language = "English (US)",
volume = "24",
pages = "671--676",
journal = "Insect Biochemistry and Molecular Biology",
issn = "0965-1748",
publisher = "Elsevier Limited",
number = "7",

}

TY - JOUR

T1 - Cloning, characterization, and genetics of the juvinile hormone esterase gene from Heliothis virescens

AU - Harshman, L. G.

AU - Ward, V. K.

AU - Beetham, J. K.

AU - Grant, D. F.

AU - Grahan, L. J.

AU - Zraket, C. A.

AU - Heckel, D. G.

AU - Hammock, B. D.

PY - 1994/7

Y1 - 1994/7

N2 - The gene for juvenile hormone esterase (JHE) was cloned from Heliothis virescens (Lepidoptera: Noctuidae). A genomic library was constructed from embryonic DNA and screened with a homologous N-terminal probe from the JHE cDNA. Five genomic clones were isolated and analyzed by dot blot hybridization using regions of the JHE cDNA as probes. Clone C hybridized to both 5′ and 3′ probes from the JHE cDNA, suggesting that clone C contains both ends of JHE gene. This was verified by sequencing the ends of the JHE gene from clone C using primers from both the 5′ and 3′ ends of the JHE cDNA. Additional sequencing and restriction mapping were used to characterized the gene. The gene is c. 8 kb long and contains four introns with consensus intron-exon junctions. One of the introns is relatively large (4 kb) and is situated near the extreme 5′ end of the gene. Genetic analysis of RFLP variation in interspecific and intraspecific crosses shows that the JHE locus is single-copy with no closely related paralogs and is autosomally encoded in Heliothis. Therefore the developmental pattern of expression of this gene and the previously documented sequence variation in cDNA clones is not explainable by reference to a JHE gene family with distinct structural loci for the different forms.

AB - The gene for juvenile hormone esterase (JHE) was cloned from Heliothis virescens (Lepidoptera: Noctuidae). A genomic library was constructed from embryonic DNA and screened with a homologous N-terminal probe from the JHE cDNA. Five genomic clones were isolated and analyzed by dot blot hybridization using regions of the JHE cDNA as probes. Clone C hybridized to both 5′ and 3′ probes from the JHE cDNA, suggesting that clone C contains both ends of JHE gene. This was verified by sequencing the ends of the JHE gene from clone C using primers from both the 5′ and 3′ ends of the JHE cDNA. Additional sequencing and restriction mapping were used to characterized the gene. The gene is c. 8 kb long and contains four introns with consensus intron-exon junctions. One of the introns is relatively large (4 kb) and is situated near the extreme 5′ end of the gene. Genetic analysis of RFLP variation in interspecific and intraspecific crosses shows that the JHE locus is single-copy with no closely related paralogs and is autosomally encoded in Heliothis. Therefore the developmental pattern of expression of this gene and the previously documented sequence variation in cDNA clones is not explainable by reference to a JHE gene family with distinct structural loci for the different forms.

KW - Heliothis virescens

KW - Juvenile hormone esterase

KW - Juvenile hormone esterase gene

KW - Juvinile hormone

UR - http://www.scopus.com/inward/record.url?scp=0028464380&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028464380&partnerID=8YFLogxK

U2 - 10.1016/0965-1748(94)90054-X

DO - 10.1016/0965-1748(94)90054-X

M3 - Article

C2 - 7915171

AN - SCOPUS:0028464380

VL - 24

SP - 671

EP - 676

JO - Insect Biochemistry and Molecular Biology

JF - Insect Biochemistry and Molecular Biology

SN - 0965-1748

IS - 7

ER -