Cloning and sequencing the cytosine methyltransferase gene M.CviJI from Chlorella virus IL-3A

Shelly L. Shields, Dwight E. Burbank, Reingard Grabherr, James L. Van Etten

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Abstract

The Chlorella virus IL-3A gene encoding the DNA methyltransferase M.CviJI, which methylates the internal cytosine in (G/A)GC(T/C/G) sequences, was cloned and expressed in Escherichia coli. The region containing the M.CviJI gene was sequenced and a single open reading frame of 1101 by was identified that could code for a polypeptide of 367 amino acids with a predicted molecular weight of 41,864. M.CviJI contained regions of amino acids which were similar to bacterial cytosine methyltransferases. Eighteen other Chlorella viruses, of 36 tested, contained DNA sequences which hybridized to the M.CviJI gene; DNA from some, but not all, of these 18 viruses also contained 5-methylcytosine in (G/A)GC(T/C/G) sequences.

Original languageEnglish (US)
Pages (from-to)16-24
Number of pages9
JournalVirology
Volume176
Issue number1
DOIs
Publication statusPublished - May 1990

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ASJC Scopus subject areas

  • Virology

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