Cloning and sequencing of the cDNA encoding human glutaredoxin

M Rohan Fernando, Hideki Sumimoto, Hiroki Nanri, Shun ichiro Kawabata, Sadaaki Iwanaga, Shigeki Minakami, Yasuyuki Fukumaki, Koichiro Takeshige

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Glutaredoxin (thioltransferase) is a small, heat-stable protein, which is involved in thiol/disulfide exchange reactions. We have isolated a cDNA that encodes glutaredoxin from a human brain cDNA library. The encoded protein contains 106 amino acids with a calculated molecular mass of 11.76 kDa and an isoelectric point of 8.09. The amino acid sequence deduced from the cDNA is more than 80% identical to those of other mammalian glutaredoxins.

Original languageEnglish (US)
Pages (from-to)229-231
Number of pages3
JournalBBA - Gene Structure and Expression
Volume1218
Issue number2
DOIs
StatePublished - Jun 21 1994

Fingerprint

Glutaredoxins
Cloning
Organism Cloning
Complementary DNA
Amino Acids
Isoelectric Point
Molecular mass
Gene Library
Sulfhydryl Compounds
Disulfides
Amino Acid Sequence
Brain
Proteins
Hot Temperature

Keywords

  • (Human)
  • Glutaredoxin
  • Nucleotide sequence
  • Thioltransferase
  • cDNA cloning

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Structural Biology

Cite this

Fernando, M. R., Sumimoto, H., Nanri, H., Kawabata, S. I., Iwanaga, S., Minakami, S., ... Takeshige, K. (1994). Cloning and sequencing of the cDNA encoding human glutaredoxin. BBA - Gene Structure and Expression, 1218(2), 229-231. https://doi.org/10.1016/0167-4781(94)90019-1

Cloning and sequencing of the cDNA encoding human glutaredoxin. / Fernando, M Rohan; Sumimoto, Hideki; Nanri, Hiroki; Kawabata, Shun ichiro; Iwanaga, Sadaaki; Minakami, Shigeki; Fukumaki, Yasuyuki; Takeshige, Koichiro.

In: BBA - Gene Structure and Expression, Vol. 1218, No. 2, 21.06.1994, p. 229-231.

Research output: Contribution to journalArticle

Fernando, MR, Sumimoto, H, Nanri, H, Kawabata, SI, Iwanaga, S, Minakami, S, Fukumaki, Y & Takeshige, K 1994, 'Cloning and sequencing of the cDNA encoding human glutaredoxin', BBA - Gene Structure and Expression, vol. 1218, no. 2, pp. 229-231. https://doi.org/10.1016/0167-4781(94)90019-1
Fernando MR, Sumimoto H, Nanri H, Kawabata SI, Iwanaga S, Minakami S et al. Cloning and sequencing of the cDNA encoding human glutaredoxin. BBA - Gene Structure and Expression. 1994 Jun 21;1218(2):229-231. https://doi.org/10.1016/0167-4781(94)90019-1
Fernando, M Rohan ; Sumimoto, Hideki ; Nanri, Hiroki ; Kawabata, Shun ichiro ; Iwanaga, Sadaaki ; Minakami, Shigeki ; Fukumaki, Yasuyuki ; Takeshige, Koichiro. / Cloning and sequencing of the cDNA encoding human glutaredoxin. In: BBA - Gene Structure and Expression. 1994 ; Vol. 1218, No. 2. pp. 229-231.
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