Cloning and sequencing of a human pancreatic tumor mucin cDNA

M. S. Lan, Surinder Kumar Batra, W. N. Qi, R. S. Metzgar, Michael A Hollingsworth

Research output: Contribution to journalArticle

348 Citations (Scopus)

Abstract

A monospecific polyclonal antiserum against deglycosylated human pancreatic tumor mucin was used to select human pancreatic mucin cDNA clones from a λgt11 cDNA expression library developed from a human pancreatic tumor cell line. The full-length 4.4-kilobase mucin cDNA sequence included a 72-base pair 5'-untranslated region and a 307-base pair 3'-untranslated region. The predicted amino acid sequence for this cDNA revealed a protein of 122,071 daltons containing 1,255 amino acid residues of which greater than 60% were serine, threonine, proline, alanine, and glycine. Approximately two-thirds of the protein sequence consisted of identical 20-amino acid tandem repeats which were flanked by degenerate tandem repeats and nontandem repeat sequences on both the amino-terminal and carboxyl-terminal ends. The amino acid sequence also contained five putative N-linked glycosylation sites, a putative signal sequence and transmembrane domain, and numerous serine and threonine residues (potential O-linked glycosylation sites) outside and within the tandem repeat position. The cDNA and deduced amino acid sequence of the pancreatic mucin sequence was over 99% homologous with a mucin cDNA sequence derived from breast tumor mucin, even though the native forms of these molecules are quite distinct in size and degree of glycosylation.

Original languageEnglish (US)
Pages (from-to)15294-15299
Number of pages6
JournalJournal of Biological Chemistry
Volume265
Issue number25
StatePublished - Sep 28 1990
Externally publishedYes

Fingerprint

Cloning
Mucins
Organism Cloning
Tumors
Complementary DNA
Glycosylation
Tandem Repeat Sequences
Amino Acids
Amino Acid Sequence
Neoplasms
Threonine
Base Pairing
Serine
5' Untranslated Regions
3' Untranslated Regions
Protein Sorting Signals
Tumor Cell Line
Gene Library
Proline
Alanine

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Cloning and sequencing of a human pancreatic tumor mucin cDNA. / Lan, M. S.; Batra, Surinder Kumar; Qi, W. N.; Metzgar, R. S.; Hollingsworth, Michael A.

In: Journal of Biological Chemistry, Vol. 265, No. 25, 28.09.1990, p. 15294-15299.

Research output: Contribution to journalArticle

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