Cloning and expression of an α-amylase gene from Phanerochaete chrysosporium

Bo Wu, Guo Ku Hu, Hong Feng, Jin Ming Wu, Yi Zheng Zhang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Based on the genomic sequence and cDNA library screening, the cDNA sequence encoding an α-amylase was cloned from the filamentous white-rot fungus Phanerochaete chrysosporium and designated as pcamy1. Alignment results showed that the predicted protein has up to 43% amino acid homology to the known α-amylases in other organisms and is close to those from some filamentous fungi. Under nitrogen-starvation condition, the transcription of pcamy1 was accordingly upregulated or downregulated when soluble starch or glucose is sole carbon source. Addition of oxygen to nitrogen-limited media led to pcamy1 transcription and removal of glucose metabolic repression. The result indicated that the pcamy1 transcript was not only regulated by nutrients such as the carbon source but also by the cultivation environment, such as oxygen. This coordinate-regulatory model is likely common in P. chrysosporium. The expressed product of this gene in Escherichia coli could hydrolyze soluble starch, and its enzymatic activity was determined. As far as we know, this is the first report about cloning and expression study on the α-amylase in P. chrysosporium.

Original languageEnglish (US)
Pages (from-to)105-113
Number of pages9
JournalCurrent Microbiology
Volume55
Issue number2
DOIs
StatePublished - Aug 1 2007

Fingerprint

Phanerochaete
Amylases
Organism Cloning
Gene Expression
Starch
Fungi
Nitrogen
Carbon
Oxygen
Glucose
Genomic Library
Starvation
Gene Library
Down-Regulation
Complementary DNA
Escherichia coli
Amino Acids
Food
Genes
Proteins

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

Cite this

Cloning and expression of an α-amylase gene from Phanerochaete chrysosporium. / Wu, Bo; Hu, Guo Ku; Feng, Hong; Wu, Jin Ming; Zhang, Yi Zheng.

In: Current Microbiology, Vol. 55, No. 2, 01.08.2007, p. 105-113.

Research output: Contribution to journalArticle

Wu, Bo ; Hu, Guo Ku ; Feng, Hong ; Wu, Jin Ming ; Zhang, Yi Zheng. / Cloning and expression of an α-amylase gene from Phanerochaete chrysosporium. In: Current Microbiology. 2007 ; Vol. 55, No. 2. pp. 105-113.
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