Cloning and characterization of the promoter region of a gene encoding a 67-kDa glycoprotein

Nabendu Chatterjeer, Cheng Zou, John C. Osterman, Naba K. Gupta

Research output: Contribution to journalArticle

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Abstract

A rat genomic library constructed in λ-EMBL3 (SP6/T7) vector (CLONTECH) was screened using 32p-labeled rat p67 cDNA. A clone containing a segment of 5'-upstream region of p67 genomic DNA was obtained. The DNA (about 1.7 kilobase pairs) was isolated and characterized. Sequence analysis of this DNA fragment showed that the 898 base pairs at the 5'-end of the upstream region was identical to several long interspersed nucleotide sequences. One hundred forty-eight base pairs at the 3'-end contained the beginning of the first exon including the ATG initiator codon. The remaining 652 base pairs in between contained two AT-rich regions and several regulatory sequences. The mRNA initiation site was identified at 89 base pairs upstream from the translation start codon. The DNA fragment was also analyzed by transient transfection. When linked to a firefly luciferase reporter gene, this fragment enhanced transcription in a rat hepatoma cell line (KRC-7). Using a series of deletions in the DNA, the minimum essential promoter region (from - 177 to -60) was identified. The promoter activity was also enhanced by treatment with phorbol 13-myristate 12-acetate (PMA). This enhancement required an AP-1 sequence (-298 to -292; 5'-TGACTCA-3') and a similar sequence (-97 to -88; 5'-ATGACATCAT-3'). Deletion of either of these sequences significantly reduced PMA enhancement. Deletion of both of these sequences almost completely eliminated PMA enhancement.

Original languageEnglish (US)
Pages (from-to)12692-12698
Number of pages7
JournalJournal of Biological Chemistry
Volume272
Issue number19
DOIs
StatePublished - May 9 1997

Fingerprint

Gene encoding
Cloning
Genetic Promoter Regions
Base Pairing
Organism Cloning
Glycoproteins
Myristic Acid
Acetates
Initiator Codon
Sequence Deletion
DNA
Rats
Genes
AT Rich Sequence
Firefly Luciferases
Genomic Library
Transcription Factor AP-1
DNA Sequence Analysis
Reporter Genes
Transfection

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Cloning and characterization of the promoter region of a gene encoding a 67-kDa glycoprotein. / Chatterjeer, Nabendu; Zou, Cheng; Osterman, John C.; Gupta, Naba K.

In: Journal of Biological Chemistry, Vol. 272, No. 19, 09.05.1997, p. 12692-12698.

Research output: Contribution to journalArticle

Chatterjeer, Nabendu ; Zou, Cheng ; Osterman, John C. ; Gupta, Naba K. / Cloning and characterization of the promoter region of a gene encoding a 67-kDa glycoprotein. In: Journal of Biological Chemistry. 1997 ; Vol. 272, No. 19. pp. 12692-12698.
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