Clonal heterogeneity of the sensitivity of human colon carcinoma cell lines to TGFβ isoforms

Guo‐Hao K. Zhou, Gwendolyn L. Sechrist, Michael G. Brattain, Kathleen M. Mulder

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Spontaneously arising, TGFβ1‐resistant colonies were isolated directly from the soft agarose plates of MOSER human colon carcinoma cells grown in the presence of TGFβ1 but in the absence of serum. The colonies were cloned by limiting dilution and screened in a monolayer proliferation assay for sensitivity to TGFβ1 and TGFβ2 isoforms. Cell clones selectively sensitive or resistant to these isoforms in the growth inhibition assay displayed similar differential sensitivities to TGFβ isoforms for production of the extracellular matrix proteins laminin and fibronectin, as well as for the expression of the colon cell differentiation marker carcinoembryonic antigen. Differential receptor binding profiles for TGFβ1 and TGFβ2 were observed among the clones. The isolation of cell clones selectively resistant or sensitive to TGFβ isoforms as well as the identification of differential receptor binding profiles among the clones indicate the heterogeneity of TGFβ responsiveness that exists naturally in human colon tumor cells and stress the importance of defining mechanisms underlying differential responsiveness to TGFβ isoforms. © 1995 Wiley‐Liss Inc.

Original languageEnglish (US)
Pages (from-to)512-520
Number of pages9
JournalJournal of Cellular Physiology
Volume165
Issue number3
DOIs
StatePublished - Dec 1995

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Protein Isoforms
Colon
Cells
Differentiation Antigens
Carcinoma
Clone cells
Cell Line
Clone Cells
Assays
Cell Separation
Extracellular Matrix Proteins
Carcinoembryonic Antigen
Laminin
Fibronectins
Sepharose
Dilution
Tumors
Cell Differentiation
Monolayers
Growth

ASJC Scopus subject areas

  • Physiology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Clonal heterogeneity of the sensitivity of human colon carcinoma cell lines to TGFβ isoforms. / Zhou, Guo‐Hao K.; Sechrist, Gwendolyn L.; Brattain, Michael G.; Mulder, Kathleen M.

In: Journal of Cellular Physiology, Vol. 165, No. 3, 12.1995, p. 512-520.

Research output: Contribution to journalArticle

Zhou, Guo‐Hao K. ; Sechrist, Gwendolyn L. ; Brattain, Michael G. ; Mulder, Kathleen M. / Clonal heterogeneity of the sensitivity of human colon carcinoma cell lines to TGFβ isoforms. In: Journal of Cellular Physiology. 1995 ; Vol. 165, No. 3. pp. 512-520.
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