Clinical applications of capillary electrophoresis based immunoassays

Annette C Moser, Corey W. Willicott, David S Hage

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Immunoassays have long been an important set of tools in clinical laboratories for the detection, diagnosis, and treatment of disease. Over the last two decades, there has been growing interest in utilizing CE as a means for conducting immunoassays with clinical samples. The resulting method is known as a CE immunoassay. This approach makes use of the selective and strong binding of antibodies for their targets, as is employed in a traditional immunoassay, and combines this with the speed, efficiency, and small sample requirements of CE. This review discusses the variety of ways in which CE immunoassays have been employed with clinical samples. An overview of the formats and detection modes that have been employed in these applications is first presented. A more detailed discussion is then given on the type of clinical targets and samples that have been measured or studied by using CE immunoassays. Particular attention is given to the use of this method in the fields of endocrinology, pharmaceutical measurements, protein and peptide analysis, immunology, infectious disease detection, and oncology. Representative applications in each of these areas are described, with these examples involving work with both traditional and microanalytical CE systems.

Original languageEnglish (US)
Pages (from-to)937-955
Number of pages19
JournalELECTROPHORESIS
Volume35
Issue number7
DOIs
StatePublished - Jan 1 2014

Fingerprint

Capillary electrophoresis
Capillary Electrophoresis
Immunoassay
Endocrinology
Clinical laboratories
Immunology
Oncology
Peptides
Antibodies
Pharmaceutical Preparations
Proteins
Clinical Laboratory Techniques
Allergy and Immunology
Communicable Diseases

Keywords

  • Affinity capillary electrophoresis
  • CE immunoassay
  • Capillary electrophoresis
  • Clinical chemistry
  • Immunoassay

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Clinical applications of capillary electrophoresis based immunoassays. / Moser, Annette C; Willicott, Corey W.; Hage, David S.

In: ELECTROPHORESIS, Vol. 35, No. 7, 01.01.2014, p. 937-955.

Research output: Contribution to journalArticle

@article{c1e9e8882fdc4d68b88cc895b2020e81,
title = "Clinical applications of capillary electrophoresis based immunoassays",
abstract = "Immunoassays have long been an important set of tools in clinical laboratories for the detection, diagnosis, and treatment of disease. Over the last two decades, there has been growing interest in utilizing CE as a means for conducting immunoassays with clinical samples. The resulting method is known as a CE immunoassay. This approach makes use of the selective and strong binding of antibodies for their targets, as is employed in a traditional immunoassay, and combines this with the speed, efficiency, and small sample requirements of CE. This review discusses the variety of ways in which CE immunoassays have been employed with clinical samples. An overview of the formats and detection modes that have been employed in these applications is first presented. A more detailed discussion is then given on the type of clinical targets and samples that have been measured or studied by using CE immunoassays. Particular attention is given to the use of this method in the fields of endocrinology, pharmaceutical measurements, protein and peptide analysis, immunology, infectious disease detection, and oncology. Representative applications in each of these areas are described, with these examples involving work with both traditional and microanalytical CE systems.",
keywords = "Affinity capillary electrophoresis, CE immunoassay, Capillary electrophoresis, Clinical chemistry, Immunoassay",
author = "Moser, {Annette C} and Willicott, {Corey W.} and Hage, {David S}",
year = "2014",
month = "1",
day = "1",
doi = "10.1002/elps.201300421",
language = "English (US)",
volume = "35",
pages = "937--955",
journal = "Electrophoresis",
issn = "0173-0835",
publisher = "Wiley-VCH Verlag",
number = "7",

}

TY - JOUR

T1 - Clinical applications of capillary electrophoresis based immunoassays

AU - Moser, Annette C

AU - Willicott, Corey W.

AU - Hage, David S

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Immunoassays have long been an important set of tools in clinical laboratories for the detection, diagnosis, and treatment of disease. Over the last two decades, there has been growing interest in utilizing CE as a means for conducting immunoassays with clinical samples. The resulting method is known as a CE immunoassay. This approach makes use of the selective and strong binding of antibodies for their targets, as is employed in a traditional immunoassay, and combines this with the speed, efficiency, and small sample requirements of CE. This review discusses the variety of ways in which CE immunoassays have been employed with clinical samples. An overview of the formats and detection modes that have been employed in these applications is first presented. A more detailed discussion is then given on the type of clinical targets and samples that have been measured or studied by using CE immunoassays. Particular attention is given to the use of this method in the fields of endocrinology, pharmaceutical measurements, protein and peptide analysis, immunology, infectious disease detection, and oncology. Representative applications in each of these areas are described, with these examples involving work with both traditional and microanalytical CE systems.

AB - Immunoassays have long been an important set of tools in clinical laboratories for the detection, diagnosis, and treatment of disease. Over the last two decades, there has been growing interest in utilizing CE as a means for conducting immunoassays with clinical samples. The resulting method is known as a CE immunoassay. This approach makes use of the selective and strong binding of antibodies for their targets, as is employed in a traditional immunoassay, and combines this with the speed, efficiency, and small sample requirements of CE. This review discusses the variety of ways in which CE immunoassays have been employed with clinical samples. An overview of the formats and detection modes that have been employed in these applications is first presented. A more detailed discussion is then given on the type of clinical targets and samples that have been measured or studied by using CE immunoassays. Particular attention is given to the use of this method in the fields of endocrinology, pharmaceutical measurements, protein and peptide analysis, immunology, infectious disease detection, and oncology. Representative applications in each of these areas are described, with these examples involving work with both traditional and microanalytical CE systems.

KW - Affinity capillary electrophoresis

KW - CE immunoassay

KW - Capillary electrophoresis

KW - Clinical chemistry

KW - Immunoassay

UR - http://www.scopus.com/inward/record.url?scp=84897433662&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84897433662&partnerID=8YFLogxK

U2 - 10.1002/elps.201300421

DO - 10.1002/elps.201300421

M3 - Article

VL - 35

SP - 937

EP - 955

JO - Electrophoresis

JF - Electrophoresis

SN - 0173-0835

IS - 7

ER -