Class-specific restrictions define primase interactions with DNA template and replicative helicase

Marilynn A Larson, Mark A Griep, Rafael Bressani, Kiran Chintakayala, Panos Soultanas, Steven Heye Hinrichs

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Bacterial primase is stimulated by replicative helicase to produce RNA primers that are essential for DNA replication. To identify mechanisms regulating primase activity, we characterized primase initiation specificity and interactions with the replicative helicase for gram-positive Firmicutes (Staphylococcus, Bacillus and Geobacillus) and gram-negative Proteobacteria (Escherichia, Yersinia and Pseudomonas). Contributions of the primase zinc-binding domain, RNA polymerase domain and helicase-binding domain on de novo primer synthesis were determined using mutated, truncated, chimeric and wild-type primases. Key residues in the β4 strand of the primase zinc-binding domain defined class-associated trinucleotide recognition and substitution of these amino acids transferred specificity across classes. A change in template recognition provided functional evidence for interaction in trans between the zinc-binding domain and RNA polymerase domain of two separate primases. Helicase binding to the primase C-terminal helicase-binding domain modulated RNA primer length in a species-specific manner and productive interactions paralleled genetic relatedness. Results demonstrated that primase template specificity is conserved within a bacterial class, whereas the primase-helicase interaction has co-evolved within each species.

Original languageEnglish (US)
Pages (from-to)7167-7178
Number of pages12
JournalNucleic acids research
Volume38
Issue number20
DOIs
StatePublished - Nov 1 2010

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DNA Primase
DNA
Zinc
DNA-Directed RNA Polymerases
Geobacillus
RNA Helicases
Escherichia
Yersinia
Proteobacteria
Amino Acid Substitution
Pseudomonas
DNA Replication
Staphylococcus
Bacillus

ASJC Scopus subject areas

  • Genetics

Cite this

Class-specific restrictions define primase interactions with DNA template and replicative helicase. / Larson, Marilynn A; Griep, Mark A; Bressani, Rafael; Chintakayala, Kiran; Soultanas, Panos; Hinrichs, Steven Heye.

In: Nucleic acids research, Vol. 38, No. 20, 01.11.2010, p. 7167-7178.

Research output: Contribution to journalArticle

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