Class I MHC molecules

Assembly and antigen presentation

Research output: Contribution to journalReview article

66 Citations (Scopus)

Abstract

Several years ago, the only factor known to be necessary for the assembly and surface expression of class I MHC was β2m; even for β2m, it was unclear at what point in class I maturation its role was played. Recent experiments that employed attachment of an endoplasmic reticulum (ER) retention signal to β2m have shown that the point of time at which β2m is required is while the class I heavy chain is in the ER. Later association between β2m and class I is not vital in order for properly folded class I to be expressed at the cell surface. After crystallization of the first class I MHC molecule, it was realized that not only is antigen presented by class I, but that antigen is presented in the form of a peptide that stabilizes the class I structure and allows its transit to the cell surface. Class I allelic differences influence interactions with both peptide and β2m, with likely consequences for the ability of the class I heavy chains to present antigen through alternative pathways. Furthermore, it is now also clear that formation of appropriate disulfide bonds in the class I heavy chain is needed before class I can bind peptide antigen securely, a process that may be assisted by an ER chaperone. Many different proteins that are resident in the ER, such as calnexin, transporter associated with antigen processing (TAP), calreticulin, and tapasin, have been found to be integral to class I assembly. TAP, tapasin, and calreticulin bind preferentially to the open form of class I, which can be distinguished with the use of a monoclonal antibody specific for this form. Calreticulin and calnexin contrast in their interactions with class I, despite other similarities between these two chaperones. Overall, class I MHC assembly is now understood to involve the interplay of multiple intra- and intermolecular events in a defined chronological order which ensure continual reporting of cellular contents to cytotoxic T lymphocytes.

Original languageEnglish (US)
Pages (from-to)11-19
Number of pages9
JournalImmunological Reviews
Volume172
DOIs
StatePublished - Jan 1 1999

Fingerprint

Antigen Presentation
Calreticulin
Endoplasmic Reticulum
Calnexin
Antigens
Peptides
Histocompatibility Antigens Class I
Cytotoxic T-Lymphocytes
Crystallization
Disulfides
Monoclonal Antibodies
Proteins
tapasin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Class I MHC molecules : Assembly and antigen presentation. / Solheim, Joyce C.

In: Immunological Reviews, Vol. 172, 01.01.1999, p. 11-19.

Research output: Contribution to journalReview article

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