Cigarette smoking behaviors and time since quitting are associated with differential DNA methylation across the human genome

Emily S. Wan, Weiliang Qiu, Andrea Baccarelli, Vincent J. Carey, Helene Bacherman, Stephen I. Rennard, Alvar Agusti, Wayne Anderson, David A. Lomas, Dawn L. Demeo

Research output: Contribution to journalArticle

189 Citations (Scopus)

Abstract

The impact of cigarette smoking can persist for extended periods following smoking cessation and may involve epigenetic reprogramming. Changes in DNA methylation associated with smoking may help to identify molecular pathways that contribute to the latency between exposure and disease onset. Cross-sectional cohort data from subjects in the International COPD Genetics Network (n = 1085) and the Boston Early-Onset COPD study (n = 369) were analyzed as the discovery and replication cohorts, respectively. Genome-wide methylation data on 27 578 CpG sites in 14 475 genes were obtained on DNA from peripheral blood leukocytes using the Illumina HumanMethylation27K Beadchip in both cohorts. We identified 15 sites significantly associated with current smoking, 2 sites associated with cumulative smoke exposure, and, within the subset of former smokers, 3 sites associated with time since quitting cigarettes. Two loci, factor II receptor-like 3 (F2RL3) and G-protein-coupled receptor 15 (GPR15), were significantly associated in all three analyses and were validated by pyrosequencing. These findings (i) identify a novel locus (GPR15) associated with cigarette smoking and (ii) suggest the existence of dynamic, site-specific methylation changes in response to smoking which may contribute to the extended risks associated with cigarette smoking that persist after cessation.

Original languageEnglish (US)
Article numberdds135
Pages (from-to)3073-3082
Number of pages10
JournalHuman Molecular Genetics
Volume21
Issue number13
DOIs
StatePublished - Jul 1 2012

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Human Genome
DNA Methylation
Smoking
G-Protein-Coupled Receptors
Chronic Obstructive Pulmonary Disease
Methylation
Smoking Cessation
Epigenomics
Smoke
Tobacco Products
Leukocytes
Genome
DNA
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Wan, E. S., Qiu, W., Baccarelli, A., Carey, V. J., Bacherman, H., Rennard, S. I., ... Demeo, D. L. (2012). Cigarette smoking behaviors and time since quitting are associated with differential DNA methylation across the human genome. Human Molecular Genetics, 21(13), 3073-3082. [dds135]. https://doi.org/10.1093/hmg/dds135

Cigarette smoking behaviors and time since quitting are associated with differential DNA methylation across the human genome. / Wan, Emily S.; Qiu, Weiliang; Baccarelli, Andrea; Carey, Vincent J.; Bacherman, Helene; Rennard, Stephen I.; Agusti, Alvar; Anderson, Wayne; Lomas, David A.; Demeo, Dawn L.

In: Human Molecular Genetics, Vol. 21, No. 13, dds135, 01.07.2012, p. 3073-3082.

Research output: Contribution to journalArticle

Wan, ES, Qiu, W, Baccarelli, A, Carey, VJ, Bacherman, H, Rennard, SI, Agusti, A, Anderson, W, Lomas, DA & Demeo, DL 2012, 'Cigarette smoking behaviors and time since quitting are associated with differential DNA methylation across the human genome', Human Molecular Genetics, vol. 21, no. 13, dds135, pp. 3073-3082. https://doi.org/10.1093/hmg/dds135
Wan, Emily S. ; Qiu, Weiliang ; Baccarelli, Andrea ; Carey, Vincent J. ; Bacherman, Helene ; Rennard, Stephen I. ; Agusti, Alvar ; Anderson, Wayne ; Lomas, David A. ; Demeo, Dawn L. / Cigarette smoking behaviors and time since quitting are associated with differential DNA methylation across the human genome. In: Human Molecular Genetics. 2012 ; Vol. 21, No. 13. pp. 3073-3082.
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