Cigarette smoke stimulates release of neutrophil chemotactic activity from cultured bovine bronchial epithelial cells

S. Shoji, R. F. Ertl, S. Koyama, R. Robbins, G. Leikauf, Susanna G Von Essen, S. I. Rennard

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

1. Recruitment of neutrophils into the airway is a prominent feature of chronic bronchitis, a syndrome often associated with exposure to cigarette smoke. Since bronchial epithelial cells are the 'first' lung cells to come into contact with smoke, these cells may be responsible for neutrophil recruitment into the airway by release of neutrophil chemotactic activity in response to cigarette smoke. 2. To evaluate this hypothesis, we prepared bovine bronchial epithelial cells and measured their ability to release neutrophil chemotactic activity following exposure to cigarette smoke. Bronchial epithelial cells were prepared by overnight digestion with protease, filtered through 100-μm Nitex mesh and resuspended in Dulbecco's modified Eagle medium supplemented with 10% fetal calf serum and antibiotics and cultured at 2x106 cells in 2ml of medium in 35-mm culture dishes. After 4 days, dishes were rinsed and refed with medium without fetal calf serum and incubated with and without 1:10 diluted smoke extract for 6h at 37°C. The neutrophil chemotactic activity of the supernatant fluids was measured by the blindwell chamber technique. 3. Cigarette smoke itself added to medium did not stimulate chemotaxis. In contrast, cigarette smoke did stimulate the release of neutrophil chemotactic activity from bovine bronchial epithelial cells [15±3 (control) versus 74±5 (smoke), P<0.01]. 4. This neutrophil chemotactic activity was dialysable, pepsin and acid stable, heat sensitive and lipid extractable, Sephadex G-75 column chromatography demonstrated two peaks of neutrophil chemotactic activity. 5. The lipoxygenase inhibitors diethylcarbamazine and nordihydroguaratic acid both diminished the release of chemotactic activity, suggesting that the activity may be a lipoxygenase product(s). 6. Dibutyryl cyclic AMP also blocked the smoke stimulated release of neutrophil chemotactic activity, suggesting that its release may be regulated by intracellular cyclic AMP. 7. Thus, bovine bronchial epithelial cells release neutrophil chemotactic activity in response to cigarette smoke, suggesting that bronchial epithelial cells may be modulators of the airway inflammatory response caused by cigarette smoke.

Original languageEnglish (US)
Pages (from-to)337-344
Number of pages8
JournalClinical Science
Volume88
Issue number3
DOIs
StatePublished - Jan 1 1995

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Smoke
Tobacco Products
Neutrophils
Epithelial Cells
Neutrophil Infiltration
Diethylcarbamazine
Lipoxygenase Inhibitors
Bucladesine
Eagles
Acids
Aptitude
Lipoxygenase
Pepsin A
Chronic Bronchitis
Chemotaxis
Serum
Cyclic AMP
Chromatography
Digestion
Peptide Hydrolases

Keywords

  • Airway epithelial cells
  • Cigarette smoke
  • Neutrophil chemotaxis

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Cigarette smoke stimulates release of neutrophil chemotactic activity from cultured bovine bronchial epithelial cells. / Shoji, S.; Ertl, R. F.; Koyama, S.; Robbins, R.; Leikauf, G.; Von Essen, Susanna G; Rennard, S. I.

In: Clinical Science, Vol. 88, No. 3, 01.01.1995, p. 337-344.

Research output: Contribution to journalArticle

Shoji, S. ; Ertl, R. F. ; Koyama, S. ; Robbins, R. ; Leikauf, G. ; Von Essen, Susanna G ; Rennard, S. I. / Cigarette smoke stimulates release of neutrophil chemotactic activity from cultured bovine bronchial epithelial cells. In: Clinical Science. 1995 ; Vol. 88, No. 3. pp. 337-344.
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