Cigarette smoke inhibits osteogenic differentiation and proliferation of human osteoprogenitor cells in monolayer and three-dimensional collagen gel culture

Xiang-de Liu, Y. K. Zhu, T. Umino, J. R. Spurzem, Debra Romberger, H. Wang, Elizabeth Cecile Reed, Stephen Israel Rennard

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Cigarette smoke is a risk factor not only for emphysema but also for other disorders characterized by deficient tissue repair, including osteoporosis. We hypothesized, therefore, that smoke might directly impair bone cell repair processes. To evaluate this, bone marrow osteoprogenitor cells were isolated from normal subjects and cultured in monolayer and in three-dimensional type I collagen gel culture. Human osteoprogenitor cells could be induced to differentiate toward osteoblast-like cells in both culture conditions by osteogenic supplements. Under both culture conditions, cigarette smoke extract (CSE) inhibited the proliferation of osteoprogenitor cells in a concentration-dependent manner. CSE also inhibited differentiation of osteoprogenitor cells toward osteoblast-like cells as assayed by alkaline phosphatase activity and calcium incorporation into cell layer. Cells in monolayer culture were more sensitive to the effect of smoke than cells in three-dimensional gel culture. Similar results were obtained with osteoblast-like cells derived from osteosarcomas. This study, therefore, demonstrates that cigarette smoke may affect bone progenitor cells directly and in this manner may contribute to the development of osteoporosis.

Original languageEnglish (US)
Pages (from-to)208-219
Number of pages12
JournalJournal of Laboratory and Clinical Medicine
Volume137
Issue number3
DOIs
StatePublished - Jan 1 2001

Fingerprint

Smoke
Tobacco Products
Monolayers
Collagen
Gels
Osteoblasts
Cell culture
Bone
Repair
Osteoporosis
Collagen Type I
Bone and Bones
Alkaline Phosphatase
Emphysema
Osteosarcoma
Cells
Bone Marrow Cells
Tissue
Calcium
Cell Differentiation

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Cigarette smoke inhibits osteogenic differentiation and proliferation of human osteoprogenitor cells in monolayer and three-dimensional collagen gel culture. / Liu, Xiang-de; Zhu, Y. K.; Umino, T.; Spurzem, J. R.; Romberger, Debra; Wang, H.; Reed, Elizabeth Cecile; Rennard, Stephen Israel.

In: Journal of Laboratory and Clinical Medicine, Vol. 137, No. 3, 01.01.2001, p. 208-219.

Research output: Contribution to journalArticle

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AU - Reed, Elizabeth Cecile

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