Cigarette smoke inhibits lung fibroblast proliferation and chemotaxis

Y. Nakamura, Debra Romberger, L. Tate, R. F. Ertl, M. Kawamoto, Y. Adachi, T. Mio, Joseph Harold Sisson, J. R. Spurzem, S. I. Rennard

Research output: Contribution to journalArticle

148 Citations (Scopus)

Abstract

Cigarette smoking is the most clearly recognized cause of pulmonary emphysema. Since loss of lung tissue, which characterizes emphysema, represents a balance between injury and repair, we hypothesized that cigarette smoke might contribute to the development of emphysema by inhibiting fibroblast proliferation and migration. To evaluate this, we examined the effect of cigarette smoke extract (CSE) on the proliferation and migration of human lung fibroblasts in vitro. CSE inhibited fibroblast proliferation and migration at noncytotoxic concentrations. When CSE was treated to remove volatile components, it showed less inhibitory activity on fibroblast proliferation. Therefore, we also examined acrolein and acetaldehyde, which are volatile components of cigarette smoke. Micromolar concentrations of acrolein and millimolar concentrations of acetaldehyde induced significant inhibition of fibroblast proliferation. In contrast, removal of volatile components did not eliminate the inhibitory activity of CSE for fibroblast migration, although acetaldehyde and acrolein alone were also capable of inhibiting chemotaxis. Cigarette smoke-induced inhibition of fibroblast proliferation and migration may impair lung repair following lung injury, and may thus contribute to the development of pulmonary emphysema.

Original languageEnglish (US)
Pages (from-to)1497-1503
Number of pages7
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume151
Issue number5
DOIs
StatePublished - May 1995

Fingerprint

Chemotaxis
Smoke
Tobacco Products
Fibroblasts
Lung
Acrolein
Acetaldehyde
Pulmonary Emphysema
Emphysema
Lung Injury
Smoking
Wounds and Injuries

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

Cite this

Cigarette smoke inhibits lung fibroblast proliferation and chemotaxis. / Nakamura, Y.; Romberger, Debra; Tate, L.; Ertl, R. F.; Kawamoto, M.; Adachi, Y.; Mio, T.; Sisson, Joseph Harold; Spurzem, J. R.; Rennard, S. I.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 151, No. 5, 05.1995, p. 1497-1503.

Research output: Contribution to journalArticle

Nakamura, Y, Romberger, D, Tate, L, Ertl, RF, Kawamoto, M, Adachi, Y, Mio, T, Sisson, JH, Spurzem, JR & Rennard, SI 1995, 'Cigarette smoke inhibits lung fibroblast proliferation and chemotaxis', American Journal of Respiratory and Critical Care Medicine, vol. 151, no. 5, pp. 1497-1503. https://doi.org/10.1164/ajrccm.151.5.7735606
Nakamura, Y. ; Romberger, Debra ; Tate, L. ; Ertl, R. F. ; Kawamoto, M. ; Adachi, Y. ; Mio, T. ; Sisson, Joseph Harold ; Spurzem, J. R. ; Rennard, S. I. / Cigarette smoke inhibits lung fibroblast proliferation and chemotaxis. In: American Journal of Respiratory and Critical Care Medicine. 1995 ; Vol. 151, No. 5. pp. 1497-1503.
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