Cigarette smoke induces interleukin-8 release from human bronchial epithelial cells

Tadashi Mio, Debra Romberger, Austin Bassett Thompson, Richard A. Robbins, Art Heires, Stephen I. Rennard

Research output: Contribution to journalArticle

315 Citations (Scopus)

Abstract

Cigarette smoking causes the development of chronic bronchitis and chronic obstructive pulmonary disease. We hypothesized that exposure to cigarette smoke might initiate release of inflammatory mediators by bronchial epithelial cells. To evaluate this, the effect of cigarette smoke extract (CSE) on IL-8 release from cultured human bronchial epithelial cells was examined. CSE augmented IL-8 release from bronchial epithelial cells in a concentration- and time-dependent manner. Most of the augmenting activity of CSE on IL-8 release from bronchial epithelial cells was lost after volatilization or lyophilization treatment. Two major volatile factors in cigarette smoke, acrolein and acetaldehyde, augmented IL-8 release. Four cell strains were tested and showed increased IL-8 release in response to CSE. In addition, bronchoalveolar lavage was performed on 11 nonsmokers and 12 smokers. IL-8 concentration was greater in the proximal, bronchial samples than in distal, alveolar samples, and IL-8 in BAL from smokers was higher than in BAL from nonsmokers. There was a significant correlation between IL- 8 concentration and neutrophil count in bronchial samples of BAL fluid. These data support the hypothesis that exposure to cigarette smoke may induce bronchial epithelial cells to release IL-8 and that this may contribute to airway inflammation in smokers.

Original languageEnglish (US)
Pages (from-to)1770-1776
Number of pages7
JournalAmerican Journal of Respiratory and Critical Care Medicine
Volume155
Issue number5
DOIs
StatePublished - Jan 1 1997

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Interleukin-8
Smoke
Tobacco Products
Epithelial Cells
Dimercaprol
Acrolein
Volatilization
Freeze Drying
Acetaldehyde
Chronic Bronchitis
Bronchoalveolar Lavage
Chronic Obstructive Pulmonary Disease
Neutrophils
Smoking
Inflammation

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Critical Care and Intensive Care Medicine

Cite this

Cigarette smoke induces interleukin-8 release from human bronchial epithelial cells. / Mio, Tadashi; Romberger, Debra; Thompson, Austin Bassett; Robbins, Richard A.; Heires, Art; Rennard, Stephen I.

In: American Journal of Respiratory and Critical Care Medicine, Vol. 155, No. 5, 01.01.1997, p. 1770-1776.

Research output: Contribution to journalArticle

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