Chronic nicotine exposure exacerbates transient focal cerebral ischemiainduced brain injury

Chun Li, Hong Sun, Denise M. Arrick, William G. Mayhan

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Tobacco smoking is a risk factor contributing to the development and progression of ischemic stroke. Among many chemicals in tobacco, nicotine may be a key contributor. We hypothesized that nicotine alters the balance between oxidant and antioxidant networks leading to an increase in brain injury following transient focal cerebral ischemia. Male Sprague-Dawley were treated with nicotine (2 or 4 mg•kg1•day1) for 4 wk via an implanted subcutaneous osmotic minipump and subjected to a 2-h middle cerebral artery occlusion (MCAO). Infarct size and neurological deficits were evaluated at 24 h of reperfusion. Superoxide levels were determined by lucigenin-enhanced chemiluminescence. Expression of oxidant and antioxidant proteins was measured using Western blot analysis. We found that chronic nicotine exposure significantly increased infarct size and worsened neurological deficits. In addition, nicotine significantly elevated superoxide levels of cerebral cortex under basal conditions. Transient focal cerebral ischemia produced an increase in superoxide levels of cerebral cortex in control group, but no further increase was found in the nicotine group. Furthermore, chronic nicotine exposure did not alter protein expression of NADPH oxidase but significantly decreased MnSOD and uncoupling protein-2 (UCP-2) in the cerebral cortex and cerebral arteries. Our findings suggest that nicotine-induced exacerbation in brain damage following transient focal cerebral ischemia may be related to a preexisting oxidative stress via decreasing of MnSOD and UCP-2.

Original languageEnglish (US)
Pages (from-to)328-333
Number of pages6
JournalJournal of Applied Physiology
Volume120
Issue number3
DOIs
StatePublished - Feb 1 2016

Fingerprint

Nicotine
Brain Injuries
Transient Ischemic Attack
Superoxides
Cerebral Cortex
Oxidants
Antioxidants
Cerebral Arteries
NADPH Oxidase
Middle Cerebral Artery Infarction
Luminescence
Reperfusion
Tobacco
Proteins
Oxidative Stress
Western Blotting
Smoking
Stroke
Control Groups
Brain

Keywords

  • Ischemia
  • MnSOD
  • Nicotine
  • Oxidative stress
  • Reperfusion
  • UCP-2

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Chronic nicotine exposure exacerbates transient focal cerebral ischemiainduced brain injury. / Li, Chun; Sun, Hong; Arrick, Denise M.; Mayhan, William G.

In: Journal of Applied Physiology, Vol. 120, No. 3, 01.02.2016, p. 328-333.

Research output: Contribution to journalArticle

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