Chronic Ethanol‐Induced Impairments in Receptor‐Mediated Endocytosis of Insulin in Rat Hepatocytes

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Abstract

The effects of chronic ethanol administration on the receptor‐mediated endocytosis of insulin were investigated in isolated hepatocytes. When hepatocytes were isolated from rats that were fed an ethanol liquid diet for 5‐6 weeks, these cells bound 25% less insulin to their surface membrane than did cells from the chow‐fed or pairfed controls. This decreased binding was likely a result of reduced surface receptor number rather than changes in receptor affinity. Rates of insulin degradation were also reduced by 25‐30% in hepatocytes from the ethanol‐fed animals. In addition, chronic ethanol feeding induced a defect in the internalization of the receptor‐insulin complex and altered the hepatocellular processing of the internalized insulin. These results indicate that chronic ethanol administration impairs both the surface binding and the endocytosis of insulin by the liver.

Original languageEnglish (US)
Pages (from-to)808-813
Number of pages6
JournalAlcoholism: Clinical and Experimental Research
Volume15
Issue number5
DOIs
StatePublished - Oct 1991

Fingerprint

Endocytosis
Rats
Hepatocytes
Insulin
Ethanol
Nutrition
Liver
Animals
Cell Membrane
Diet
Membranes
Degradation
Defects
Liquids
Processing

Keywords

  • Chronic Ethanol Feeding
  • Hepatic Receptor‐mediated Endocytosis
  • Insulin

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology
  • Psychiatry and Mental health

Cite this

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title = "Chronic Ethanol‐Induced Impairments in Receptor‐Mediated Endocytosis of Insulin in Rat Hepatocytes",
abstract = "The effects of chronic ethanol administration on the receptor‐mediated endocytosis of insulin were investigated in isolated hepatocytes. When hepatocytes were isolated from rats that were fed an ethanol liquid diet for 5‐6 weeks, these cells bound 25{\%} less insulin to their surface membrane than did cells from the chow‐fed or pairfed controls. This decreased binding was likely a result of reduced surface receptor number rather than changes in receptor affinity. Rates of insulin degradation were also reduced by 25‐30{\%} in hepatocytes from the ethanol‐fed animals. In addition, chronic ethanol feeding induced a defect in the internalization of the receptor‐insulin complex and altered the hepatocellular processing of the internalized insulin. These results indicate that chronic ethanol administration impairs both the surface binding and the endocytosis of insulin by the liver.",
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AB - The effects of chronic ethanol administration on the receptor‐mediated endocytosis of insulin were investigated in isolated hepatocytes. When hepatocytes were isolated from rats that were fed an ethanol liquid diet for 5‐6 weeks, these cells bound 25% less insulin to their surface membrane than did cells from the chow‐fed or pairfed controls. This decreased binding was likely a result of reduced surface receptor number rather than changes in receptor affinity. Rates of insulin degradation were also reduced by 25‐30% in hepatocytes from the ethanol‐fed animals. In addition, chronic ethanol feeding induced a defect in the internalization of the receptor‐insulin complex and altered the hepatocellular processing of the internalized insulin. These results indicate that chronic ethanol administration impairs both the surface binding and the endocytosis of insulin by the liver.

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