Chronic ethanol consumption results in deficient bone repair in rats

Dennis A. Chakkalakal, Jerzy R. Novak, Edward D. Fritz, Teresa J. Mollner, Daniel L. McVicker, Denise L. Lybarger, Michael H. McGuire, Terrence Donohue

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

There is evidence that ethanol inhibits osteoblast function and that chronic ethanol consumption induces systemic bone loss and increases the risk of fracture in humans. The purpose of the present study was to determine whether chronic ethanol consumption also compromises the healing of injured bone. Male Sprague-Dawley rats, 8-10 weeks old, were placed into four feeding groups: group A received ethanol (36% of calories) as part of a liquid diet; group B was pair-fed to group A and received an isocaloric control diet containing maltodextrin; group C was fed the AIN-93M standard semi-purified liquid diet ad libitum; group D was fed the same ethanol diet as group A before bone injury, but after surgery (see below) these rats were given isocaloric control diet ad libitum. After 6 weeks on their respective diets, a bone repair model was surgically created at the midshaft in both fibulae of each rat. Seven weeks after injury the animals were euthanized and bone healing was evaluated by determining rigidity of the fibula by three-point bending, flexural modulus of the repair tissue and mineral content of the repair tissue. Rigidity of fibula in ethanol-fed rats and their pair-fed controls (groups A and B) were respectively 48 and 47% lower than in group C. Flexural modulus of the repair tissue in ethanol-fed rats had a 55% (P = 0.046) deficiency compared with their pair-fed controls. The mineral contents in groups A and B were respectively 16 and 13% lower than in group C. There were no significant differences in the results between groups C and D. Thus, the outcome of bone repair in ethanol-fed rats was deficient compared with rats receiving a standard maintenance diet. The repair tissue in ethanol-fed rats was mechanically inferior to that in pair-fed controls. This deficiency could not be attributed to the reduced food consumption of these animals. On the other hand, the restoration of normal bone healing in group D cannot be attributed solely to the cessation of ethanol feeding after bone injury because of the increased food consumption during this period.

Original languageEnglish (US)
Pages (from-to)13-20
Number of pages8
JournalAlcohol and Alcoholism
Volume37
Issue number1
StatePublished - Mar 7 2002

Fingerprint

Rats
Bone
Repair
Ethanol
Nutrition
Bone and Bones
Diet
Fibula
Tissue
Rigidity
Minerals
Wounds and Injuries
Animals
Food
Osteoblasts
Liquids
Surgery
Restoration
Sprague Dawley Rats
Maintenance

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology
  • Psychiatry and Mental health

Cite this

Chakkalakal, D. A., Novak, J. R., Fritz, E. D., Mollner, T. J., McVicker, D. L., Lybarger, D. L., ... Donohue, T. (2002). Chronic ethanol consumption results in deficient bone repair in rats. Alcohol and Alcoholism, 37(1), 13-20.

Chronic ethanol consumption results in deficient bone repair in rats. / Chakkalakal, Dennis A.; Novak, Jerzy R.; Fritz, Edward D.; Mollner, Teresa J.; McVicker, Daniel L.; Lybarger, Denise L.; McGuire, Michael H.; Donohue, Terrence.

In: Alcohol and Alcoholism, Vol. 37, No. 1, 07.03.2002, p. 13-20.

Research output: Contribution to journalArticle

Chakkalakal, DA, Novak, JR, Fritz, ED, Mollner, TJ, McVicker, DL, Lybarger, DL, McGuire, MH & Donohue, T 2002, 'Chronic ethanol consumption results in deficient bone repair in rats', Alcohol and Alcoholism, vol. 37, no. 1, pp. 13-20.
Chakkalakal DA, Novak JR, Fritz ED, Mollner TJ, McVicker DL, Lybarger DL et al. Chronic ethanol consumption results in deficient bone repair in rats. Alcohol and Alcoholism. 2002 Mar 7;37(1):13-20.
Chakkalakal, Dennis A. ; Novak, Jerzy R. ; Fritz, Edward D. ; Mollner, Teresa J. ; McVicker, Daniel L. ; Lybarger, Denise L. ; McGuire, Michael H. ; Donohue, Terrence. / Chronic ethanol consumption results in deficient bone repair in rats. In: Alcohol and Alcoholism. 2002 ; Vol. 37, No. 1. pp. 13-20.
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