Chronic ethanol administration impairs atp-dependent acidification of endosomes in rat liver

Carol A Casey, R. L. Wiegert, D. J. Tuma

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Ethanol administration has been shown to alter various protein trafficking pathways in the liver, including protein secretion, plasma membrane assembly and receptor-mediated endocytosis. Molecules such as proteins are transported into and within the cells via endosomal vesicles, and acidification of these endosomes appears to be critical for efficient sorting and trafficking of proteins. In the present study we examined whether ethanol administration impaired the ATP-dependent acidification of endosomes. Liver endosomes were prepared from rats after intravenous labeling with fluorescently labeled ligands and the initial pH and extent of ATP-dependent acidification was determined. Our results showed that the intrinsic pH was significantly higher and that the ATP-dependent acidification of the interior of the endosomes was inhibited in ethanol-fed versus control animals. This impairment of endosomal acidification may play a role in the impaired protein trafficking which occurs as a result of ethanol administration.

Original languageEnglish (US)
Pages (from-to)1127-1133
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume195
Issue number2
DOIs
Publication statusPublished - Sep 15 1993

    Fingerprint

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this